Duplicated SP11 genes produce alternative transcripts in the S15 haplotype of Brassica oleracea

Hiroshi Shiba, Jong In Park, Go Suzuki, Masanori Matsushita, Ill Sup Nou, Akira Isogai, Seiji Takayama, Masao Watanabe

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Self-incompatibility (SI) discriminating self and non-self pollen is regulated by S-locus genes in Brassica. In most S haplotypes, a set of three highly polymorphic genes, SLG, SRK, and SP11, is located at the S-locus region. In the present study, we found duplicated SP11 genes, S15-SP11a, S15-SP11b, and S15-SP11b', in the self-incompatible S 15 haplotype of B. oleracea. RNA gel blot and reverse transcription polymerase chain reaction (RT-PCR) analyses showed that two different sizes of SP11 transcripts were specifically detected in anther tissues: a 0.65-kb transcript corresponded to S15-SP11a (an exon-1 region of S 15-SP11b was also cotranscribed in some cases), and a 1.4-kb transcript contained the duplicated three genes, S15-SP11a, S 15-SP11b, and an exon-1 region of S15-SP11b', all three of which were connected to intergenic spacer regions.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalGenes and Genetic Systems
Volume79
Issue number2
DOIs
Publication statusPublished - 2004 Apr
Externally publishedYes

Keywords

  • Alternative transcript
  • Brassica oleracea
  • Gene duplication
  • S locus protein 11 (SP11)
  • Self-incompatibility (SI)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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