Dual-reporter phenotypic assay for human immunodeficiency viruses

Keiko Kajiwara, Eiichi Kodama, Yasuko Sakagami, Takeshi Naito, Masao Matsuoka

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


We have established a novel human immunodeficiency virus (HIV) tandem-reporter assay using HIV receptor-transduced NP-2 cells with long terminal repeat-controlled β-galactosidase, inserted internal ribosome entry site, and secretary alkaline phosphatase genes. This assay allows users to detect replication of clinical isolates, indicating its useful application as an HIV phenotypic assay.

Original languageEnglish
Pages (from-to)792-795
Number of pages4
JournalJournal of Clinical Microbiology
Issue number2
Publication statusPublished - 2008 Feb
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)


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