Dual mode regulation of migration by lysophosphatidic acid in human gastric cancer cells

Dai Shida, Joji Kitayama, Hironori Yamaguchi, Kotaro Hama, Junken Aoki, Hiroyuki Arai, Hiroharu Yamashita, Ken Mori, Akihiro Sako, Tsuyoshi Konishi, Toshiaki Watanabe, Teruyuki Sakai, Rika Suzuki, Hideo Ohta, Yoh Takuwa, Hirokazu Nagawa

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)


Lysophosphatidic acid (LPA), which interacts with at least three G protein-coupled receptors (GPCRs), LPA1/Edg-2, LPA2/Edg-4, and LPA3/Edg-7, is a lipid mediator with diverse effects on various cells. Here, we investigated the expression profiles of LPA receptors and patterns of LPA-induced migration in gastric cancer cells. Northern blot analysis revealed that various gastric cancer cells expressed variable levels of LPA1, LPA2, and LPA3 without a consistent pattern. Using a Boyden chamber assay, LPA markedly increased cell migration of LPA1-expressing cells, the effects of which were almost totally abrogated by Ki16425, an LPA antagonist against LPA1 and LPA3. In contrast, LPA by itself did not significantly induce migration in MKN28 and MKN74 cells, which exclusively expressed LPA2. However, when hepatocyte growth factor (HGF) was placed with LPA in the lower chamber, LPA induced migration of these cells in a dose-dependent manner. Immunoprecipitation analysis revealed that LPA induced transient tyrosine phosphorylation of c-Met in LPA2-expressing cells, which suggests that the transactivation of c-Met by LPA causes a cooperative migratory response with HGF to these cells. Our results indicate that LPA regulates the migration of gastric cancer cells in a receptor-specific manner and suggest that the expression pattern of LPA receptors may affect the metastatic behavior of gastric cancer.

Original languageEnglish
Pages (from-to)168-178
Number of pages11
JournalExperimental Cell Research
Issue number2
Publication statusPublished - 2004 Dec 10
Externally publishedYes


  • Gastric cancer cell
  • Hepatocyte growth factor
  • LPA receptor
  • Lysophosphatidic acid
  • Migration
  • Transactivation
  • c-Met

ASJC Scopus subject areas

  • Cell Biology


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