TY - JOUR
T1 - Dual comprehensive approach to decipher the Drosophila Toll pathway, ex vivo RNAi screenings and immunoprecipitation-mass spectrometry
AU - Kanoh, Hirotaka
AU - Kato, Hiroyuki
AU - Suda, Yamato
AU - Hori, Aki
AU - Kurata, Shoichiro
AU - Kuraishi, Takayuki
N1 - Funding Information:
This work was supported by grants from the Japan Science and Technology Agency for T.K.; the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) for S.K. and T.K; the Takeda Science Foundation for T.K.
Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - The Drosophila Toll pathway is involved in embryonic development, innate immunity, and cell-cell interactions. However, compared to the mammalian Toll-like receptor innate immune pathway, its intracellular signaling mechanisms are not fully understood. We have previously performed a series of ex vivo genome-wide RNAi screenings to identify genes required for the activation of the Toll pathway. In this study, we have conducted an additional genome-wide RNAi screening using the overexpression of Tube, an adapter molecule in the Toll pathway, and have performed a co-immunoprecipitation assay to identify components present in the dMyd88-Tube complex. Based on the results of these assays, we have performed a bioinformatic analysis, and describe candidate molecules and post-translational modifications that could be involved in Drosophila Toll signaling.
AB - The Drosophila Toll pathway is involved in embryonic development, innate immunity, and cell-cell interactions. However, compared to the mammalian Toll-like receptor innate immune pathway, its intracellular signaling mechanisms are not fully understood. We have previously performed a series of ex vivo genome-wide RNAi screenings to identify genes required for the activation of the Toll pathway. In this study, we have conducted an additional genome-wide RNAi screening using the overexpression of Tube, an adapter molecule in the Toll pathway, and have performed a co-immunoprecipitation assay to identify components present in the dMyd88-Tube complex. Based on the results of these assays, we have performed a bioinformatic analysis, and describe candidate molecules and post-translational modifications that could be involved in Drosophila Toll signaling.
KW - Drosophila
KW - Innate immunity
KW - Toll pathway
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U2 - 10.1016/j.bbrc.2018.11.007
DO - 10.1016/j.bbrc.2018.11.007
M3 - Article
C2 - 30497778
AN - SCOPUS:85057165815
VL - 508
SP - 332
EP - 337
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -