Dual comprehensive approach to decipher the Drosophila Toll pathway, ex vivo RNAi screenings and immunoprecipitation-mass spectrometry

Hirotaka Kanoh, Hiroyuki Kato, Yamato Suda, Aki Hori, Shoichiro Kurata, Takayuki Kuraishi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The Drosophila Toll pathway is involved in embryonic development, innate immunity, and cell-cell interactions. However, compared to the mammalian Toll-like receptor innate immune pathway, its intracellular signaling mechanisms are not fully understood. We have previously performed a series of ex vivo genome-wide RNAi screenings to identify genes required for the activation of the Toll pathway. In this study, we have conducted an additional genome-wide RNAi screening using the overexpression of Tube, an adapter molecule in the Toll pathway, and have performed a co-immunoprecipitation assay to identify components present in the dMyd88-Tube complex. Based on the results of these assays, we have performed a bioinformatic analysis, and describe candidate molecules and post-translational modifications that could be involved in Drosophila Toll signaling.

Original languageEnglish
Pages (from-to)332-337
Number of pages6
JournalBiochemical and biophysical research communications
Volume508
Issue number1
DOIs
Publication statusPublished - 2019 Jan 1

Keywords

  • Drosophila
  • Innate immunity
  • Toll pathway

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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