Drosophila arginine methyltransferase 1 (DART1) is an ecdysone receptor co-repressor

Shuhei Kimura, Shun Sawatsubashi, Saya Ito, Alexander Kouzmenko, Eriko Suzuki, Yue Zhao, Kaoru Yamagata, Masahiko Tanabe, Takashi Ueda, Sari Fujiyama, Takuya Murata, Hiroyuki Matsukawa, Ken ichi Takeyama, Nobuo Yaegashi, Shigeaki Kato

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22 Citations (Scopus)


Histone arginine methylation is an epigenetic marker that regulates gene expression by defining the chromatin state. Arginine methyltransferases, therefore, serve as transcriptional co-regulators. However, unlike other transcriptional co-regulators, the physiological roles of arginine methyltransferases are poorly understood. Drosophila arginine methyltransferase 1 (DART1), the mammalian PRMT1 homologue, methylates the arginine residue of histone H4 (H4R3me2). Disruption of DART1 in Drosophila by imprecise P-element excision resulted in low viability during metamorphosis in the pupal stages. In the pupal stage, an ecdysone hormone signal is critical for developmental progression. DART1 interacted with the nuclear ecdysone receptor (EcR) in a ligand-dependent manner, and co-repressed EcR in intact flies. These findings suggest that DART1, a histone arginine methyltransferase, is a co-repressor of EcR that is indispensable for normal pupal development in the intact fly.

Original languageEnglish
Pages (from-to)889-893
Number of pages5
JournalBiochemical and biophysical research communications
Issue number4
Publication statusPublished - 2008 Jul 11


  • Arginine methyltransferase
  • Chromatin
  • Co-repressor
  • DART1
  • Drosophila melanogaster
  • Ecdysone receptor
  • Histone modification
  • Metamorphosis
  • Transcription

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Kimura, S., Sawatsubashi, S., Ito, S., Kouzmenko, A., Suzuki, E., Zhao, Y., Yamagata, K., Tanabe, M., Ueda, T., Fujiyama, S., Murata, T., Matsukawa, H., Takeyama, K. I., Yaegashi, N., & Kato, S. (2008). Drosophila arginine methyltransferase 1 (DART1) is an ecdysone receptor co-repressor. Biochemical and biophysical research communications, 371(4), 889-893. https://doi.org/10.1016/j.bbrc.2008.05.003