Droplet array on local redox cycling-based electrochemical (LRC-EC) chip device

Kosuke Ino, Takehito Goto, Yusuke Kanno, Kumi Y. Inoue, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

We have previously reported a local redox cycling-based electrochemical (LRC-EC) system for the incorporation of many electrochemical sensors into a small chip device. In the present study, a new type of LRC-EC chip device was fabricated for the detection of a droplet array. To detect electrochemically redox compounds in droplets, Pt pseudo-reference/counter electrodes were incorporated into the individual sensors of the LRC-EC chip device. Cyclic voltammetry for the LRC-EC chip device with internal Pt pseudo-reference electrodes indicated well-defined voltammograms based on redox cycling for the individual sensor points. The device was successfully applied to the addressable detection of alkaline phosphatase (ALP) activity of HeLa cells in single droplets on the sensor points. Therefore, the LRC-EC chip device is considered to be a useful device for the bioanalysis of droplet systems.

Original languageEnglish
Pages (from-to)787-794
Number of pages8
JournalLab on a Chip
Volume14
Issue number4
DOIs
Publication statusPublished - 2014 Feb 21

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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