Down-regulation of telomerase activity in DLD-1 human colorectal adenocarcinoma cells by tocotrienol

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Abstract

As high telomerase activity is detected in most cancer cells, inhibition of telomerase by drug or dietary food components is a new strategy for cancer prevention. Here, we investigated the inhibitory effect of vitamin E, with particular emphasis on tocotrienol (unsaturated vitamin E), on human telomerase in cell-culture study. As results, tocotrienol inhibited telomerase activity of DLD-1 human colorectal adenocarcinoma cells in time- and dose-dependent manner, interestingly, with δ-tocotrienol exhibiting the highest inhibitory activity. Tocotrienol inhibited protein kinase C activity, resulting in down-regulation of c-myc and human telomerase reverse transcriptase (hTERT) expression, thereby reducing telomerase activity. In contrast to tocotrienol, tocopherol showed very weak telomerase inhibition. These results provide novel evidence for the first time indicating that tocotrienol acts as a potent candidate regulator of telomerase and supporting the anti-proliferative function of tocotrienol.

Original languageEnglish
Pages (from-to)170-175
Number of pages6
JournalBiochemical and biophysical research communications
Volume348
Issue number1
DOIs
Publication statusPublished - 2006 Sep 15

Keywords

  • Protein kinase C
  • Telomerase inhibition
  • Tocotrienol
  • c-myc
  • hTERT

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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