Down-regulation of Runx1 expression by TCR signal involves an autoregulatory mechanism and contributes to IL-2 production

Won Fen Wong, Mineo Kurokawa, Masanobu Satake, Kazuyoshi Kohu

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Runx1 transcription factor plays multiple roles in T cell development, differentiation, and function. However, the regulatory mechanisms and functional significance of high Runx1 protein expression in resting peripheral CD4+ T cells is not well understood. Here, we demonstrate that T-cell receptor (TCR) activation down-regulates distal Runx1 transcription, resulting in a significant reduction of Runx1 protein. Interestingly, this downregulation of distal Runx1 transcription appears to be mediated through a negative auto-regulatory mechanism, whereby Runx1 protein binds to a Runx consensus site in the distal promoter. Through the use of Runx1-overexpressing cells from transgenic mice, we demonstrate that interference with TCR-mediated Runx1 down-regulation inhibits IL-2 production and proliferation in activated CD4+ T cells. In contrast, using Runx1-deficient cells prepared from targeted mice, we show that the absence of Runx1 in unstimulated CD4+ T cells results in IL-2 derepression. In summary, we propose that high levels of Runx1 in resting CD4+ T cells functions negatively in the regulation of IL-2 transcription, and that TCR activation-mediated downregulation of Runx1 involves negative auto-regulation of the distal Runx1 promoter and contributes to IL-2 production.

Original languageEnglish
Pages (from-to)11110-11118
Number of pages9
JournalJournal of Biological Chemistry
Volume286
Issue number13
DOIs
Publication statusPublished - 2011 Apr 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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