Down-regulation of antigen-specific antibody production by TCR antagonist peptides in vivo

Masako Toda, Mamoru Totsuka, Shigetada Furukawa, Kahori Yokota, Tohru Yoshioka, Akio Ametani, Shuichi Kaminogawa

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The efficacy of TCR antagonist peptides in inhibition of antigen-specific antibody production and T cell responses in vivo was evaluated. Among amino acid-substituted analogs of a peptide corresponding to residues 119-133 of bovine β-lactoglobulin (p119-133), pR124Q and pD129S, prepared by substitution of Gln and Ser for Arg124 and Asp129, respectively, have been shown to display TCR antagonist activity for three out of four distinct p119-133-specific T cell clones and for polyclonal T cells derived from p119-133-immunized C57BL/6 mice. Both pD129S and pR124Q inhibited in vivo priming and subsequent activation of T cells by p119-133 when co-injected with p119-133 into mice, as shown by the decreased proliferation of T cells in response to p119-133 in vitro. pD129S significantly inhibited production of anti-p119-113 antibodies of IgG1, IgG2b and IgE isotype in vivo when co-injected into mice together with p119-133 at the time of the first immunization. However, pR124Q was totally ineffective in inhibition of the antibody responses. Anti-p119-133 antibodies from p119-133-immunized mice could bind to pR124Q but not to pD129S, suggesting that the difference in cross-reactivity is responsible for the different effect of these two peptides on specific antibody production. Our findings demonstrate that a single TCR antagonist peptide can inhibit antigen-specific polyclonal antibody production when this antagonist peptide does not cross-react with the antibody elicited in response to an antigenic peptide.

Original languageEnglish
Pages (from-to)403-414
Number of pages12
JournalEuropean Journal of Immunology
Volume30
Issue number2
DOIs
Publication statusPublished - 2000

Keywords

  • Cross-reactivity
  • IgE production
  • Immunointervention
  • Specific Ab response
  • TCR antagonist

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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