TY - JOUR
T1 - Down-regulation of antigen-specific antibody production by TCR antagonist peptides in vivo
AU - Toda, Masako
AU - Totsuka, Mamoru
AU - Furukawa, Shigetada
AU - Yokota, Kahori
AU - Yoshioka, Tohru
AU - Ametani, Akio
AU - Kaminogawa, Shuichi
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2000
Y1 - 2000
N2 - The efficacy of TCR antagonist peptides in inhibition of antigen-specific antibody production and T cell responses in vivo was evaluated. Among amino acid-substituted analogs of a peptide corresponding to residues 119-133 of bovine β-lactoglobulin (p119-133), pR124Q and pD129S, prepared by substitution of Gln and Ser for Arg124 and Asp129, respectively, have been shown to display TCR antagonist activity for three out of four distinct p119-133-specific T cell clones and for polyclonal T cells derived from p119-133-immunized C57BL/6 mice. Both pD129S and pR124Q inhibited in vivo priming and subsequent activation of T cells by p119-133 when co-injected with p119-133 into mice, as shown by the decreased proliferation of T cells in response to p119-133 in vitro. pD129S significantly inhibited production of anti-p119-113 antibodies of IgG1, IgG2b and IgE isotype in vivo when co-injected into mice together with p119-133 at the time of the first immunization. However, pR124Q was totally ineffective in inhibition of the antibody responses. Anti-p119-133 antibodies from p119-133-immunized mice could bind to pR124Q but not to pD129S, suggesting that the difference in cross-reactivity is responsible for the different effect of these two peptides on specific antibody production. Our findings demonstrate that a single TCR antagonist peptide can inhibit antigen-specific polyclonal antibody production when this antagonist peptide does not cross-react with the antibody elicited in response to an antigenic peptide.
AB - The efficacy of TCR antagonist peptides in inhibition of antigen-specific antibody production and T cell responses in vivo was evaluated. Among amino acid-substituted analogs of a peptide corresponding to residues 119-133 of bovine β-lactoglobulin (p119-133), pR124Q and pD129S, prepared by substitution of Gln and Ser for Arg124 and Asp129, respectively, have been shown to display TCR antagonist activity for three out of four distinct p119-133-specific T cell clones and for polyclonal T cells derived from p119-133-immunized C57BL/6 mice. Both pD129S and pR124Q inhibited in vivo priming and subsequent activation of T cells by p119-133 when co-injected with p119-133 into mice, as shown by the decreased proliferation of T cells in response to p119-133 in vitro. pD129S significantly inhibited production of anti-p119-113 antibodies of IgG1, IgG2b and IgE isotype in vivo when co-injected into mice together with p119-133 at the time of the first immunization. However, pR124Q was totally ineffective in inhibition of the antibody responses. Anti-p119-133 antibodies from p119-133-immunized mice could bind to pR124Q but not to pD129S, suggesting that the difference in cross-reactivity is responsible for the different effect of these two peptides on specific antibody production. Our findings demonstrate that a single TCR antagonist peptide can inhibit antigen-specific polyclonal antibody production when this antagonist peptide does not cross-react with the antibody elicited in response to an antigenic peptide.
KW - Cross-reactivity
KW - IgE production
KW - Immunointervention
KW - Specific Ab response
KW - TCR antagonist
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U2 - 10.1002/1521-4141(200002)30:2<403::AID-IMMU403>3.0.CO;2-8
DO - 10.1002/1521-4141(200002)30:2<403::AID-IMMU403>3.0.CO;2-8
M3 - Article
C2 - 10671195
AN - SCOPUS:0033955929
VL - 30
SP - 403
EP - 414
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 2
ER -