TY - JOUR
T1 - Down-regulation of an ankyrin repeat-containing protein, V-1, during skeletal muscle differentiation and its re-expression in the regenerative process of muscular dystrophy
AU - Furukawa, Yuko
AU - Hashimoto, Naohiro
AU - Yamakuni, Tohru
AU - Ishida, Yukisato
AU - Kato, Chieko
AU - Ogashiwa, Masayo
AU - Kobayashi, Masumi
AU - Kobayashi, Takayoshi
AU - Nonaka, Ikuya
AU - Mizusawa, Hidehiro
AU - Song, Si Young
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Using Western blot analysis and immunohistochemical methods, we examined the expression of V-1, a member of the ankyrin repeat-containing protein family, during differentiation and regeneration of skeletal muscle. The expression of V-1 was high in cultured myoblasts and decreased during their differentiation into myotubes, while high expression was maintained when muscle differentiation was inhibited by treatment with basic fibroblast growth factor. Down-regulation of V-1 also occurred during in vivo muscle differentiation from embryonic to postnatal stages, reaching an undetectable level in mature skeletal muscle. In contrast, strong V-1 immunoreactivity was detected again in myoblasts and regenerating muscle fibers with a small diameter, which were observed in Duchenne muscular dystrophy and its animal model, mdx mouse. Thus, it seems that V-1 is a good marker for early stage of muscle regeneration and changes of its expression suggest that V-1 plays a role in prenatal muscle differentiation and postnatal muscle regeneration.
AB - Using Western blot analysis and immunohistochemical methods, we examined the expression of V-1, a member of the ankyrin repeat-containing protein family, during differentiation and regeneration of skeletal muscle. The expression of V-1 was high in cultured myoblasts and decreased during their differentiation into myotubes, while high expression was maintained when muscle differentiation was inhibited by treatment with basic fibroblast growth factor. Down-regulation of V-1 also occurred during in vivo muscle differentiation from embryonic to postnatal stages, reaching an undetectable level in mature skeletal muscle. In contrast, strong V-1 immunoreactivity was detected again in myoblasts and regenerating muscle fibers with a small diameter, which were observed in Duchenne muscular dystrophy and its animal model, mdx mouse. Thus, it seems that V-1 is a good marker for early stage of muscle regeneration and changes of its expression suggest that V-1 plays a role in prenatal muscle differentiation and postnatal muscle regeneration.
KW - Ankyrin repeat
KW - Duchenne muscular dystrophy
KW - Muscle differentiation
KW - Muscle regeneration
KW - V-1
KW - mdx mice
UR - http://www.scopus.com/inward/record.url?scp=0037211474&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037211474&partnerID=8YFLogxK
U2 - 10.1016/S0960-8966(02)00185-2
DO - 10.1016/S0960-8966(02)00185-2
M3 - Article
C2 - 12467730
AN - SCOPUS:0037211474
VL - 13
SP - 32
EP - 41
JO - Neuromuscular Disorders
JF - Neuromuscular Disorders
SN - 0960-8966
IS - 1
ER -