Double-stranded RNA-induced interferon regulatory factor-1 gene expression in FRTL-5 rat thyroid cells

Kouki Mori, Katsumi Yoshida, Jun ichi Tani, Yoshinori Nakagawa, Saeko Hoshikawa, Sadayoshi Ito

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Double-stranded RNA (dsRNA) plays a role in the regulation of cell growth and apoptosis as well as in the cellular antiviral responses. However, it remains unknown if dsRNA-activated signaling systems are functional in the thyroid. Here we report the presence of the dsRNA-dependent protein kinase (PKR) in FRTL-5 rat thyroid cells. In poly(I)-poly(C) (pIC)-stimulated cells, activation of nuclear factor-κB (NFκB) binding was clearly induced. Incubation of FRTL-5 cells with pIC resulted in a marked increase in interferon regulatory factor-1 (IRF-1) mRNA and phosphorylated signal transducer and activator of transcription-1 (STAT1) levels. Addition of pIC to cells led to type I interferon (IFN) gene expression, especially IFNβ, which can induce STAT1 phosphorylation, suggesting that dsRNA indirectly induced STAT1 phosphorylation through expression of type I IFN. Thus, our results suggest that the dsRNA-activated signaling pathway may be involved in the regulation of IFN-inducible genes in the thyroid.

Original languageEnglish
Pages (from-to)77-86
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume184
Issue number1-2
DOIs
Publication statusPublished - 2001 Nov 26

Keywords

  • Double-stranded RNA
  • Interferon regulatory factor-1
  • Signal transduction
  • Thyroid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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