TY - JOUR
T1 - Double flower formation in Tricyrtis macranthopsis is related to low expression of AGAMOUS ortholog gene
AU - Sharifi, Ahmad
AU - Oizumi, Kanako
AU - Kubota, Shosei
AU - Bagheri, Abdolreza
AU - Shafaroudi, Saeid Malekzadeh
AU - Nakano, Masaru
AU - Kanno, Akira
N1 - Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/9/2
Y1 - 2015/9/2
N2 - Complex flowers containing large numbers of petals are especially prized as ornamental plants. Double-flowered Tricyrtis macranthopsis, in which stamens and carpels are converted to petaloid organs, is related to AGAMOUS (AG) gene expression. So we isolated AGAMOUS gene from T. macranthopsis, named as TrimAG. Since TrimAG expression was limited to whorls 3 and 4 and ectopic expression of TrimAG in Arabidopsis thaliana caused the same phenotype as overexpression of other AG orthologs in transgenic Arabidopsis, this gene has similar function as its AG orthologs. In the double-flowered T. macranthopsis cultivar, the level of TrimAG expression was reduced in the two inner reproductive whorls (stamens and carpels) and caused reproductive organ replacement with petaloid tepals and floral indeterminacy. Southern blot analysis of genomic DNA indicated that there were no significant differences between the double-flowered cultivar and wild-type plants. Analysis of promoter and AG intron II sequences did not identify any critically mutated sequences in the doubled-flowered cultivar. These results suggest that reduced TrimAG expression in whorls 3 and 4 might be caused by mutation in one of the genes in regulatory network which controls AGAMOUS expression or transcriptional silencing by methylation of TrimAG promoter/intron sequences.
AB - Complex flowers containing large numbers of petals are especially prized as ornamental plants. Double-flowered Tricyrtis macranthopsis, in which stamens and carpels are converted to petaloid organs, is related to AGAMOUS (AG) gene expression. So we isolated AGAMOUS gene from T. macranthopsis, named as TrimAG. Since TrimAG expression was limited to whorls 3 and 4 and ectopic expression of TrimAG in Arabidopsis thaliana caused the same phenotype as overexpression of other AG orthologs in transgenic Arabidopsis, this gene has similar function as its AG orthologs. In the double-flowered T. macranthopsis cultivar, the level of TrimAG expression was reduced in the two inner reproductive whorls (stamens and carpels) and caused reproductive organ replacement with petaloid tepals and floral indeterminacy. Southern blot analysis of genomic DNA indicated that there were no significant differences between the double-flowered cultivar and wild-type plants. Analysis of promoter and AG intron II sequences did not identify any critically mutated sequences in the doubled-flowered cultivar. These results suggest that reduced TrimAG expression in whorls 3 and 4 might be caused by mutation in one of the genes in regulatory network which controls AGAMOUS expression or transcriptional silencing by methylation of TrimAG promoter/intron sequences.
KW - AGAMOUS
KW - Double-flowered
KW - Intron
KW - MADS-box
KW - Promoter
KW - Tricyrtis macranthopsis
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U2 - 10.1016/j.scienta.2015.06.050
DO - 10.1016/j.scienta.2015.06.050
M3 - Article
AN - SCOPUS:84939529466
VL - 193
SP - 337
EP - 345
JO - Scientia Horticulturae
JF - Scientia Horticulturae
SN - 0304-4238
ER -