DOSEc, a heme-regulated phosphodiesterase, plays an important role in the regulation of the cyclic AMP level in Escherichia coli

Tokiko Suzuki, Ikuko Sagami, Nao Yokota, Hirofumi Kurokawa, Toru Shimizu

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Heme-regulated phosphodiesterase from Escherichia coli (DOSEc) catalyzes the hydrolysis of cyclic AMP (cAMP) in vitro and is regulated by the redox state of the bound heme. Changes in the redox state result in alterations in the three-dimensional structure of the enzyme, which is then transmitted to the functional domain to switch catalysis on or off. Because DOSEc was originally cloned from E. coli genomic DNA, it has not been known whether it is actually expressed in wild-type E. coli. In addition, the turnover number of DOSEc using cAMP as a substrate is only 0.15 min-1, which is relatively low for a physiologically relevant enzyme. In the present study, we demonstrated for the first time that the DOSEc gene and protein are expressed in wild-type E. coli, especially under aerobic conditions. We also developed a DOSEc gene knockout strain (Δdos). Interestingly, the knockout of dos caused excess accumulation of intracellular cAMP (26-fold higher than in the wild-type strain) under aerobic conditions, whereas accumulation of cAMP was not observed under anaerobic conditions. We also found differences in cell morphology and growth rate between the mutant cells and the wild-type strain. The changes in the knockout strain were partially complemented by introducing an expression plasmid for dos. Thus, the present study revealed that expression of DOSEc is regulated according to environmental O2 availability at the transcriptional level and that the concentration of cAMP in cells is regulated by DOSEc expression.

Original languageEnglish
Pages (from-to)6678-6682
Number of pages5
JournalJournal of bacteriology
Volume187
Issue number19
DOIs
Publication statusPublished - 2005 Oct 1

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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