TY - JOUR
T1 - Dopamine receptor DOP1R2 stabilizes appetitive olfactory memory through the Raf/MAPK pathway in Drosophila
AU - Sun, Huan
AU - Nishioka, Tomoki
AU - Hiramatsu, Shun
AU - Kondo, Shu
AU - Amano, Mutsuki
AU - Kaibuchi, Kozo
AU - Ichinose, Toshiharu
AU - Tanimoto, Hiromu
N1 - Funding Information:
This study was supported by Ministry of Education, Culture, Sports, Science and Technology–Japan/JSPS KAKENHI (17H05545, 17H01378, 15K14307 to H.T.; 16K20919 to T.I.; 17H01380 to K.K.) and Strategic Research ProgramforBrainSciences(H.T.,K.K.,M.A.).TheOtsukaToshimiScholarshipFoundationprovidedfinancialsupport during the study (to H.S.). We thank Nobuhiro Yamagata, Kei Ito, and Ayako Abe for technical and/or instructional supportsatinceptionstage.WealsothankTomoyukiMiyashita,YukinoriHirano,BruceA.Edgar,andMinoruSaitoe for technical assistance and/or fly sharing, some of which were not shown in this article. In addition, we thank the Janelia FlyLight Project Team, the Bloomington Drosophila Stock Center, and the Kyoto Stock Center for fly strain provision. *T.I. and H.T. contributed equally to this work. The authors declare no competing financial interests.
Funding Information:
This study was supported by Ministry of Education, Culture, Sports, Science and Technology?Japan/JSPS KAKENHI (17H05545, 17H01378, 15K14307 to H.T.; 16K20919 to T.I.; 17H01380 to K.K.) and Strategic Research Program for Brain Sciences (H.T., K.K., M.A.). The Otsuka Toshimi Scholarship Foundation provided financial support during the study (to H.S.). We thank Nobuhiro Yamagata, Kei Ito, and Ayako Abe for technical and/or instructional supports at inception stage. We also thank Tomoyuki Miyashita, Yukinori Hirano, Bruce A. Edgar, and Minoru Saitoe for technical assistance and/or fly sharing, some of which were not shown in this article. In addition, we thank the Janelia FlyLight Project Team, the Bloomington Drosophila Stock Center, and the Kyoto Stock Center for fly strain provision.
Publisher Copyright:
Copyright © 2020 the authors.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - In Drosophila, dopamine signaling to the mushroom body intrinsic neurons, Kenyon cells (KCs), is critical to stabilize olfactory memory. Little is known about the downstream intracellular molecular signaling underlying memory stabilization. Here we address this question in the context of sugar-rewarded olfactory long-term memory (LTM). We show that associative training increases the phosphorylation of MAPK in KCs, via Dop1R2 signaling. Consistently, the attenuation of Dop1R2, Raf, or MAPK expression in KCs selectively impairs LTM, but not short-term memory. Moreover, we show that the LTM deficit caused by the knockdown of Dop1R2 can be rescued by expressing active Raf in KCs. Thus, the Dop1R2/Raf/MAPK pathway is a pivotal downstream effector of dopamine signaling for stabilizing appetitive olfactory memory.
AB - In Drosophila, dopamine signaling to the mushroom body intrinsic neurons, Kenyon cells (KCs), is critical to stabilize olfactory memory. Little is known about the downstream intracellular molecular signaling underlying memory stabilization. Here we address this question in the context of sugar-rewarded olfactory long-term memory (LTM). We show that associative training increases the phosphorylation of MAPK in KCs, via Dop1R2 signaling. Consistently, the attenuation of Dop1R2, Raf, or MAPK expression in KCs selectively impairs LTM, but not short-term memory. Moreover, we show that the LTM deficit caused by the knockdown of Dop1R2 can be rescued by expressing active Raf in KCs. Thus, the Dop1R2/Raf/MAPK pathway is a pivotal downstream effector of dopamine signaling for stabilizing appetitive olfactory memory.
KW - Dopamine receptor
KW - Drosophila
KW - Memory stabilization
KW - Raf/MAPK pathway
UR - http://www.scopus.com/inward/record.url?scp=85082780317&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85082780317&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.1572-19.2020
DO - 10.1523/JNEUROSCI.1572-19.2020
M3 - Article
C2 - 32102921
AN - SCOPUS:85082780317
VL - 40
SP - 2935
EP - 2942
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 14
ER -