Donor prone positioning protects lungs from injury during warm ischemia

Yui Watanabe, Marcos Galasso, Tatsuaki Watanabe, Aadil Ali, Robert Qaqish, Daisuke Nakajima, Yohei Taniguchi, Mauricio Pipkin, Lindsay Caldarone, Manyin Chen, Takashi Kanou, Cara Summers, Khaled Ramadan, Yu Zhang, Harley Chan, Thomas K. Waddell, Mingyao Liu, Shaf Keshavjee, Lorenzo del Sorbo, Marcelo Cypel

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A large proportion of controlled donation after circulatory death (cDCD) donor lungs are declined because cardiac arrest does not occur within a suitable time after the withdrawal of life-sustaining therapy. Improved strategies to preserve lungs after asystole may allow the recovery team to arrive after death actually occurs and enable the recovery of lungs from more cDCD donors. The aim of this study was to determine the effect of donor positioning on the quality of lung preservation after cardiac arrest in a cDCD model. Cardiac arrest was induced by withdrawal of ventilation under anesthesia in pigs. After asystole, animals were divided into 2 groups based on body positioning (supine or prone). All animals were subjected to 3 hours of warm ischemia. After the observation period, donor lungs were explanted and preserved at 4°C for 6 hours, followed by 6 hours of physiologic and biological lung assessment under normothermic ex vivo lung perfusion. Donor lungs from the prone group displayed significantly greater quality as reflected by better function during ex vivo lung perfusion, less edema formation, less cell death, and decreased inflammation compared with the supine group. A simple maneuver of donor prone positioning after cardiac arrest significantly improves lung graft preservation and function.

Original languageEnglish
Pages (from-to)2746-2755
Number of pages10
JournalAmerican Journal of Transplantation
Volume19
Issue number10
DOIs
Publication statusPublished - 2019 Oct 1
Externally publishedYes

Keywords

  • animal models: porcine
  • basic (laboratory) research/science
  • donors and donation: donation after circulatory death (DCD)
  • donors and donation: extended criteria
  • lung transplantation/pulmonology
  • organ perfusion and preservation
  • organ procurement
  • organ procurement and allocation
  • translational research/science

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation
  • Pharmacology (medical)

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  • Cite this

    Watanabe, Y., Galasso, M., Watanabe, T., Ali, A., Qaqish, R., Nakajima, D., Taniguchi, Y., Pipkin, M., Caldarone, L., Chen, M., Kanou, T., Summers, C., Ramadan, K., Zhang, Y., Chan, H., Waddell, T. K., Liu, M., Keshavjee, S., del Sorbo, L., & Cypel, M. (2019). Donor prone positioning protects lungs from injury during warm ischemia. American Journal of Transplantation, 19(10), 2746-2755. https://doi.org/10.1111/ajt.15363