Dominant negative effect of a truncated erythropoietin receptor (EPOR- T) on erythropoietin-induced erythroid differentiation: Possible involvement of EPOR-T in ineffective erythropoiesis of myelodysplastic syndrome

Ritsuko Shimizu, Norio Komatsu, Yasusada Miura

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

We isolated a human leukemic cell line UT-7/GM from UT-7, which can differentiate into mature erythroid cells with erythropoietin (EPO) treatment. Using this cell line, we examined the effect of a truncated human EPO receptor (EPOR-T) on EPO-induced erythroid differentiation. Transfection studies revealed that UT-7/GM cells expressing exogenous EPOR-T were likely to undergo apoptosis even in the presence of EPO. In addition, EPOR-T- transfected cells could not differentiate into hemoglobin-positive cells after administration of EPO. These results suggest that EPOR-T is a negative regulator of EPO-induced anti-apoptosis and EPO-induced erythroid differentiation. The EPOR-T form was expressed in seven of nine cases of myelodysplastic syndrome but not in normal controls. In patients with myelodysplastic syndrome, dysregulated expression of EPOR-T may cause apoptosis and blockage of erythroid differentiation, resulting in ineffective erythropoiesis.

Original languageEnglish
Pages (from-to)229-233
Number of pages5
JournalExperimental Hematology
Volume27
Issue number2
DOIs
Publication statusPublished - 1999 Feb
Externally publishedYes

Keywords

  • Apoptosis
  • Erythropoiesis
  • Erythropoietin receptor
  • Myelodysplastic syndrome
  • UT-7

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

Fingerprint

Dive into the research topics of 'Dominant negative effect of a truncated erythropoietin receptor (EPOR- T) on erythropoietin-induced erythroid differentiation: Possible involvement of EPOR-T in ineffective erythropoiesis of myelodysplastic syndrome'. Together they form a unique fingerprint.

Cite this