Domain movements of HAP2 in the cap-filament complex formation and growth process of the bacterial flagellum

Saori Maki-Yonekura, Koji Yonekura, Keiichi Namba

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

The cap at the growing end of the bacterial flagellum is essential for its growth, remaining stably attached while permitting the insertion of flagellin transported from the cytoplasm through the narrow central channel. We analyzed the structure of the isolated cap in its frozen hydrated state by electron cryomicroscopy. The 3D density map now shows detailed features of domains and their connections, giving reliable volumes and masses, making assignment of the domains to the amino acid sequence possible. A model of the cap-filament complex built with an atomic model of the filament allows a quantitative analysis of the cap domain movements on cap binding and rotation that promotes the efficient self assembly of flagellin during the filament growth process.

Original languageEnglish
Pages (from-to)15528-15533
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number26
DOIs
Publication statusPublished - 2003 Dec 23
Externally publishedYes

ASJC Scopus subject areas

  • General

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