Dom34: Hbs1 Plays a General Role in Quality-Control Systems by Dissociation of a Stalled Ribosome at the 3' End of Aberrant mRNA

Tatsuhisa Tsuboi, Kazushige Kuroha, Kazuhei Kudo, Shiho Makino, Eri Inoue, Isao Kashima, Toshifumi Inada

Research output: Contribution to journalArticlepeer-review

158 Citations (Scopus)

Abstract

Translation arrest leads to an endonucleolytic cleavage of mRNA that is termed no-go decay (NGD). It has been reported that the Dom34:Hbs1 complex stimulates this endonucleolytic cleavage of mRNA induced by translation arrest in vivo and dissociates subunits of a stalled ribosome in vitro. Here we report that Dom34:Hbs1 dissociates the subunits of a ribosome that is stalled at the 3' end of mRNA in vivo, and has a crucial role in both NGD and nonstop decay. Dom34:Hbs1-mediated dissociation of a ribosome that is stalled at the 3' end of mRNA is required for degradation of a 5'-NGD intermediate. Dom34:Hbs1 facilitates the decay of nonstop mRNAs from the 3' end by exosomes and is required for the complete degradation of nonstop mRNA decay intermediates. We propose that Dom34:Hbs1 stimulates degradation of the 5'-NGD intermediate and of nonstop mRNA by dissociating the ribosome that is stalled at the 3' end of the mRNA.

Original languageEnglish
Pages (from-to)518-529
Number of pages12
JournalMolecular Cell
Volume46
Issue number4
DOIs
Publication statusPublished - 2012 May 25

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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