Does estrogen alter the mechanical properties of the anterior cruciate ligament? An experimental study in rabbits

Tatsuro Komatsuda, Takehiko Sugita, Hirotaka Sano, Takashi Kusakabe, Munenori Watanuki, Yusuke Yoshizumi, Takashi Murakami, Minoru Hashimoto, Shoichi Kokubun

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: It is well known that anterior cruciate ligament (ACL) injuries are commoner in female athletes. Accordingly, we hypothesized that serum estrogen may play some role in this sex difference. We evaluated the relationship between serum estrogen levels and the mechanical properties of the ACL in rabbits. Animals and methods: In 40 ovariectomized rabbits, the serum estrogen levels (SEL) were controlled by intramuscular injection of 17β-estradiol. The mean SEL in each rabbit was defined as the average of 5 determinations done at 1, 2, 3, 4 and 5 weeks after ovariectomy. The animals were divided into 4 groups according to the dose of estradiol administered (low, medium, high and control: L, M, H and C, respectively) and into 2 groups according to the mean SEL (high-SEL group and low-SEL group). The medial portions of ACL attached to both femur and tibia harvested at 5 weeks after ovariectomy were examined mechanically. Results: The mean serum estrogen levels in groups C, L, M and H were 37, 50, 60 and 231 pg/mL. Statistically significant differences in the mean serum estrogen levels were seen among the 4 groups, except between groups L and M. Statistically significant differences were found between groups M and H in both the ultimate tensile stress and linear stiffness. In the comparison between 2 groups using the mean SEL value, both ultimate tensile stress and linear stiffness were lower in the high-SEL group. In all animals, a positive correlation was found between ultimate tensile stress and linear stiffness. Interpretation: Our findings suggest that high SEL might be one of the factors in the multifactorial pathogenesis of ACL rupture. Copyright

Original languageEnglish
Pages (from-to)973-980
Number of pages8
JournalActa Orthopaedica
Volume77
Issue number6
DOIs
Publication statusPublished - 2006 Dec 1

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Fingerprint Dive into the research topics of 'Does estrogen alter the mechanical properties of the anterior cruciate ligament? An experimental study in rabbits'. Together they form a unique fingerprint.

  • Cite this

    Komatsuda, T., Sugita, T., Sano, H., Kusakabe, T., Watanuki, M., Yoshizumi, Y., Murakami, T., Hashimoto, M., & Kokubun, S. (2006). Does estrogen alter the mechanical properties of the anterior cruciate ligament? An experimental study in rabbits. Acta Orthopaedica, 77(6), 973-980. https://doi.org/10.1080/17453670610013312