Do genetic variants in the SPINK1 gene affect the level of serum PSTI?

Kiyoshi Kume, Atsushi Masamune, Hiroyuki Ariga, Shintaro Hayashi, Tetsuya Takikawa, Shin Miura, Noriaki Suzuki, Kazuhiro Kikuta, Shin Hamada, Morihisa Hirota, Atsushi Kanno, Tooru Shimosegawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background The serine protease inhibitor Kazal type 1 (SPINK1), also known as pancreatic secretory trypsin inhibitor (PSTI), is a peptide secreted by pancreatic acinar cells. Genetic studies have shown an association between SPINK1 gene variants and chronic pancreatitis or recurrent acute pancreatitis. The aim of this study was to clarify whether the SPINK1 variants affect the level of serum PSTI. Methods One hundred sixty-three patients with chronic pancreatitis or recurrent acute pancreatitis and 73 healthy controls were recruited. Serum PSTI concentrations were determined with a commercial radioimmunoassay kit. Results Ten patients with the p.N34S variant, 7 with the IVS3+2T>C variant, two with both the p.N34S and the IVS3+2T>C variants, and one with the novel missense p.P45S variant in the SPINK1 gene were identified. The serum PSTI level in patients with no SPINK1 variants was 14.3 ± 9.6 ng/ml (mean ± SD), and that in healthy controls was 10.7 ± 2.2 ng/ml. The PSTI level in patients carrying the IVS3+2T>C variant (5.1 ± 3.4 ng/ml), but not in those with the p.N34S variant (8.9 ± 3.5 ng/ml), was significantly lower than that in the patients without the SPINK1 variants and the healthy controls. The serum PSTI level in the patient with the p.P45S variant was 4.9 ng/ml. Low levels of serum PSTI (<6.0 ng/ml) showed sensitivity of 80 %, specificity of 97 %, and accuracy of 96 % in the differentiation of IVS3+2T>C and p.P45S carriers from non-carriers. Conclusion Serum PSTI levels were decreased in patients with the IVS3+2T>C and p.P45S variants of the SPINK1 gene.

Original languageEnglish
Pages (from-to)1267-1274
Number of pages8
JournalJournal of gastroenterology
Volume47
Issue number11
DOIs
Publication statusPublished - 2012 Nov

Keywords

  • Mutation
  • Pancreatic secretory trypsin inhibitor
  • Pancreatitis
  • Polymorphism
  • Serine protease inhibitor Kazal type 1

ASJC Scopus subject areas

  • Gastroenterology

Fingerprint Dive into the research topics of 'Do genetic variants in the SPINK1 gene affect the level of serum PSTI?'. Together they form a unique fingerprint.

  • Cite this

    Kume, K., Masamune, A., Ariga, H., Hayashi, S., Takikawa, T., Miura, S., Suzuki, N., Kikuta, K., Hamada, S., Hirota, M., Kanno, A., & Shimosegawa, T. (2012). Do genetic variants in the SPINK1 gene affect the level of serum PSTI? Journal of gastroenterology, 47(11), 1267-1274. https://doi.org/10.1007/s00535-012-0590-3