DNA transfection of mouse lymphoid cells by the combination of DEAE-dextran-mediated DNA uptake and osmotic shock procedure

Toshiyuki Takai, Hitoshi Ohmori

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)

Abstract

Several mouse lymphoid cell lines were efficiently transfected with plasmid DNA by a novel method combining DEAE-dextran-mediated DNA uptake and osmotic shock procedure. The cells were first incubated with DNA-DEAE-dextran complex, treated with hypertonic Tris-HCl buffer containing 0.5 M sucrose and 10% poly(ethylene glycol), and then exposed to hypotonic RPMI 1640 medium. This transfection protocol exhibited maximal frequencies of 0.3% and 3·10-5 for transient gene expression and stable transformation in P3-NSI/1-Ag4-1 cells, respectively.

Original languageEnglish
Pages (from-to)105-109
Number of pages5
JournalBBA - Gene Structure and Expression
Volume1048
Issue number1
DOIs
Publication statusPublished - 1990 Jan 30
Externally publishedYes

Keywords

  • (Mouse lymphoid cell)
  • DEAE-dextran
  • DNA transfection
  • Osmotic shock

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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