DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.

Hirofumi Sato, Yusuke Hashimoto, Mayuko Kikkawa, Takehiko Wada, Yoshihisa Inoue

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Oligomeric peptide ribonucleic acids (PRNA's), tethering 5'-amino-5'-deoxyribonucleosides as a recognition site for nucleic acids, has been designed and synthesized by solid phase method. PRNA 12-mer found to form stable duplex with complementary DNA.PRNA.DNA duplex composed of PRNA with mismatched DNA has 10-degree lower melting temperature than fullmatched PRNA.DNA duplex. These results demonstrate that binding of PRNA with DNA is sequence specific. It should be emphasized that the on-off switching of PRNA.DNA duplex formation has been realized by the borate added as an external factor.

Original languageEnglish
Pages (from-to)159-160
Number of pages2
JournalNucleic acids research. Supplement (2001)
Issue number2
Publication statusPublished - 2002 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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