DNA probes for detection of Marteilioides chungmuensis from the ovary of Pacific oyster Crassostrea gigas

Naoki Itoh, Tadashi Oda, Tomoyoshi Yoshinaga, Kazuo Ogawa

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The detection of the paramyxean parasite Marteilioides chungmuensis has been achieved only by using standard histological techniques or gross observations, which have a limited sensitivity for early diagnosis. In the present study, small subunit ribosomal DNA of the parasite was sequenced, and polymerase chain reaction (PCR) primers designed accordingly. It has been demonstrated that both the PCR and in situ hybridization techniques are specific for M. chungmuensis. Moreover, compared to traditional histological techniques and gross observation, the PCR diagnosis showed a far greater sensitivity. These new diagnostic tools based on molecular techniques can also be used for the elucidation of the parasite's life cycle and the identification of possible intermediate hosts.

Original languageEnglish
Pages (from-to)163-169
Number of pages7
JournalFish Pathology
Volume38
Issue number4
DOIs
Publication statusPublished - 2003 Dec

Keywords

  • Crassostrea gigas
  • Detection
  • In situ hybridization
  • Marteilioides chungnmuensis
  • PCR
  • Pacific oyster
  • Paramyxea
  • Parasite

ASJC Scopus subject areas

  • Aquatic Science
  • Animal Science and Zoology

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