DNA microarray analysis of human neuroblastoma SH-SY5Y cells exposed to methylmercury

Takashi Toyama, Eiko Yoshida, Yasuhiro Shinkai, Yoshito Kumagai

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12 Citations (Scopus)

Abstract

To investigate the adaptive response to the environmental electrophile methylmercury (MeHg), we performed DNA microarray analysis of human neuroblastoma SH-SY5Y cells exposed to a sub-cytotoxic dose of MeHg (1 μM) for 6 hr. The expression of 15 genes increased 10-fold or more in response to MeHg. Four of these genes are associated with detoxification and excretion of MeHg into the extracellular space, and are regulated by transcription factor Nrf2 through the electrophile response element. Interestingly, Cullin3, a negative regulator of Nrf2, was identified as a down-regulated gene during MeHg exposure.

Original languageEnglish
Pages (from-to)843-845
Number of pages3
JournalJournal of Toxicological Sciences
Volume36
Issue number6
DOIs
Publication statusPublished - 2011

Keywords

  • Methylmercury
  • Microarray analysis
  • Nrf2

ASJC Scopus subject areas

  • Toxicology

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