DNA methylation imprints on the IG-DMR of the Dlk1-Gtl2 domain in mouse male germline

Hitoshi Hiura; Junichi Komiyama; Motomu Shirai; Yayoi Obata; Hidehiko Ogawa; Tomohiro Kono

doi:10.1016/j.febslet.2007.02.034

DNA methylation imprints on the IG-DMR of the Dlk1-Gtl2 domain in mouse male germline

Hitoshi Hiura, Junichi Komiyama, Motomu Shirai, Yayoi Obata, Hidehiko Ogawa, Tomohiro Kono

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Mouse genomes show a large cluster of imprinted genes at the Dlk1-Gtl2 domain in the distal region of chromosome 12. An intergenic-differentially methylated region (IG-DMR) located between Dlk1 and Gtl2 is specifically methylated in the male germline; IG-DMR regulates the parental allele-specific expression of imprinted genes. Here, we show the resetting of IG-DMR methylation marks during male germ-cell differentiation. For parental allele-specific methylation analysis, polymorphisms were detected in a 2.6-kb IG-DMR in three mouse strains. Bisulfite methylation analysis showed erasure of the marks by E14 and re-establishment before birth. The IG-DMR methylation status was maintained in spermatogonia and spermatocytes of mature testes. The IG-DMR methylation status established before birth is thus maintained throughout the lifetime in the male germline.

Original language	English
Pages (from-to)	1255-1260
Number of pages	6
Journal	FEBS Letters
Volume	581
Issue number	7
DOIs	https://doi.org/10.1016/j.febslet.2007.02.034
Publication status	Published - 2007 Apr 3
Externally published	Yes

Keywords

DMR
Genomic imprinting
Germ cells
Methylation
Mouse

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.02.034

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title = "DNA methylation imprints on the IG-DMR of the Dlk1-Gtl2 domain in mouse male germline",

abstract = "Mouse genomes show a large cluster of imprinted genes at the Dlk1-Gtl2 domain in the distal region of chromosome 12. An intergenic-differentially methylated region (IG-DMR) located between Dlk1 and Gtl2 is specifically methylated in the male germline; IG-DMR regulates the parental allele-specific expression of imprinted genes. Here, we show the resetting of IG-DMR methylation marks during male germ-cell differentiation. For parental allele-specific methylation analysis, polymorphisms were detected in a 2.6-kb IG-DMR in three mouse strains. Bisulfite methylation analysis showed erasure of the marks by E14 and re-establishment before birth. The IG-DMR methylation status was maintained in spermatogonia and spermatocytes of mature testes. The IG-DMR methylation status established before birth is thus maintained throughout the lifetime in the male germline.",

keywords = "DMR, Genomic imprinting, Germ cells, Methylation, Mouse",

author = "Hitoshi Hiura and Junichi Komiyama and Motomu Shirai and Yayoi Obata and Hidehiko Ogawa and Tomohiro Kono",

note = "Funding Information: This work was supported by a grant from the Bio-oriented Technology Research Advancement Institution (BRAIN), Japan, and the Ministry of Education, Science, Culture and Sports of Japan (#18208024).",

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AU - Hiura, Hitoshi

AU - Komiyama, Junichi

AU - Shirai, Motomu

AU - Obata, Yayoi

AU - Ogawa, Hidehiko

AU - Kono, Tomohiro

N1 - Funding Information: This work was supported by a grant from the Bio-oriented Technology Research Advancement Institution (BRAIN), Japan, and the Ministry of Education, Science, Culture and Sports of Japan (#18208024).

PY - 2007/4/3

Y1 - 2007/4/3

N2 - Mouse genomes show a large cluster of imprinted genes at the Dlk1-Gtl2 domain in the distal region of chromosome 12. An intergenic-differentially methylated region (IG-DMR) located between Dlk1 and Gtl2 is specifically methylated in the male germline; IG-DMR regulates the parental allele-specific expression of imprinted genes. Here, we show the resetting of IG-DMR methylation marks during male germ-cell differentiation. For parental allele-specific methylation analysis, polymorphisms were detected in a 2.6-kb IG-DMR in three mouse strains. Bisulfite methylation analysis showed erasure of the marks by E14 and re-establishment before birth. The IG-DMR methylation status was maintained in spermatogonia and spermatocytes of mature testes. The IG-DMR methylation status established before birth is thus maintained throughout the lifetime in the male germline.

AB - Mouse genomes show a large cluster of imprinted genes at the Dlk1-Gtl2 domain in the distal region of chromosome 12. An intergenic-differentially methylated region (IG-DMR) located between Dlk1 and Gtl2 is specifically methylated in the male germline; IG-DMR regulates the parental allele-specific expression of imprinted genes. Here, we show the resetting of IG-DMR methylation marks during male germ-cell differentiation. For parental allele-specific methylation analysis, polymorphisms were detected in a 2.6-kb IG-DMR in three mouse strains. Bisulfite methylation analysis showed erasure of the marks by E14 and re-establishment before birth. The IG-DMR methylation status was maintained in spermatogonia and spermatocytes of mature testes. The IG-DMR methylation status established before birth is thus maintained throughout the lifetime in the male germline.

KW - DMR

KW - Genomic imprinting

KW - Germ cells

KW - Methylation

KW - Mouse

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DNA methylation imprints on the IG-DMR of the Dlk1-Gtl2 domain in mouse male germline

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Keywords

ASJC Scopus subject areas

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