DNA methylation imprints on the IG-DMR of the Dlk1-Gtl2 domain in mouse male germline

Hitoshi Hiura, Junichi Komiyama, Motomu Shirai, Yayoi Obata, Hidehiko Ogawa, Tomohiro Kono

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


Mouse genomes show a large cluster of imprinted genes at the Dlk1-Gtl2 domain in the distal region of chromosome 12. An intergenic-differentially methylated region (IG-DMR) located between Dlk1 and Gtl2 is specifically methylated in the male germline; IG-DMR regulates the parental allele-specific expression of imprinted genes. Here, we show the resetting of IG-DMR methylation marks during male germ-cell differentiation. For parental allele-specific methylation analysis, polymorphisms were detected in a 2.6-kb IG-DMR in three mouse strains. Bisulfite methylation analysis showed erasure of the marks by E14 and re-establishment before birth. The IG-DMR methylation status was maintained in spermatogonia and spermatocytes of mature testes. The IG-DMR methylation status established before birth is thus maintained throughout the lifetime in the male germline.

Original languageEnglish
Pages (from-to)1255-1260
Number of pages6
JournalFEBS Letters
Issue number7
Publication statusPublished - 2007 Apr 3
Externally publishedYes


  • DMR
  • Genomic imprinting
  • Germ cells
  • Methylation
  • Mouse

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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