DNA Methylation Changes Induced in Rice by Exposure to High Concentrations of the Nitric Oxide Modulator, Sodium Nitroprusside

Xiufang Ou, Tingting Zhuang, Wenchao Yin, Yiling Miao, Bo Wang, Yunhong Zhang, Xiuyun Lin, Chunming Xu, Diter von Wettstein, Sachin Rustgi, Bao Liu

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Nitric oxide (NO) is a signaling molecule that participates at physiological concentrations in diverse regulatory pathways. However, when NO concentration reaches levels higher than normal, it is sensed as a stress, which results in epigenetic alterations and chromatin remodeling in plants. Here, we showed that exposure to 0.5 mM of sodium nitroprusside (SNP), a NO donor, induces stress symptoms in rice seedlings that result in complete growth inhibition. Moreover, these stress symptoms in SNP-treated plants were found to be associated with methylation changes in genomic DNA, which predominantly involve hypomethylation at the CHG sites. Concomitant with these changes, transcriptional activation of a number of genes and transposable elements (TEs) was also observed. Further analysis revealed that expression of four genes, Chromomethylase 3 (OsCMT3), deficient in DNA methylation 1 (OsDDM1a), OsDDM1b, and DEMETER (OsDME), involved in chromatin remodeling and DNA methylation homoeostasis have been specifically perturbed.

Original languageEnglish
Pages (from-to)1428-1440
Number of pages13
JournalPlant Molecular Biology Reporter
Volume33
Issue number5
DOIs
Publication statusPublished - 2015 Oct 31
Externally publishedYes

Keywords

  • DNA methylation
  • Gene expression
  • Nitric oxide
  • Rice

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

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