DNA extraction and amplification methods for ephippial cases of Daphnia resting eggs in lake sediments: A novel approach for reconstructing zooplankton population structure from the past

Seiji Ishida, Hajime Ohtsuki, Tamotsu Awano, Narumi K. Tsugeki, Wataru Makino, Yoshihisa Suyama, Jotaro Urabe

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

This study describes a method of DNA extraction and amplification for ephippial cases of Daphnia resting eggs from lake sediment. Recent studies have reconstructed succession records of Daphnia species by genetically analyzing Daphnia resting eggs stored in lake sediments and revealed changes in dominant Daphnia species that correspond well with environmental changes. However, this approach is not applicable to lakes where most of the resting eggs in the sediment have already hatched out. We modified conventional methods for DNA extraction and amplification to enable genetic analyses of the ephippial case that envelops and protects the resting eggs, and we compared the performance of the modified method to the conventional one. We confirmed that we could efficiently analyze the sequences of the ephippial cases collected in a sediment core using our modified method. It enables us to reconstruct changes in genetic structure of Daphnia populations regardless of hatching rates of the resting eggs.

Original languageEnglish
Pages (from-to)261-267
Number of pages7
JournalLimnology
Volume13
Issue number3
DOIs
Publication statusPublished - 2012 Dec

Keywords

  • DNA extraction
  • Daphnia
  • Ephippia
  • Paleolimnology
  • Resting eggs

ASJC Scopus subject areas

  • Ecology
  • Aquatic Science
  • Water Science and Technology

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