DNA-dependent protein kinase activity correlates with Ku70 expression and radiation sensitivity in esophageal cancer cell lines

Heng Jiang Zhao, Yoshio Hosoi, Hideo Miyachi, Keiichiro Ishii, Masayuki Yoshida, Kenji Nemoto, Yoshihiro Takai, Shogo Yamada, Norio Suzuki, Tetsuya Ono

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

We investigated the relationship between DNA-dependent protein kinase (DNA-PK) activity and radiation sensitivity using 14 esophageal cancer cell lines, TE 1-14. DNA-PK activities differed significantly among the cell lines. The highest DNA-PK activity observed in TE-8 was more than two times higher than the lowest DNA-PK activity observed in TE-5. Significant correlation was observed between DNA-PK activity and DO (r = 0.766; P = 0.0008), Western blots analysis revealed a significant correlation between DNA-PK activity and Ku70 expression, suggesting that the regulation in DNA-PK activity was associated with Ku70 expression. The data suggest that the measurement of DNA-PK activity and/or Ku70 expression may provide a useful way to predict radiation sensitivity.

Original languageEnglish
Pages (from-to)1073-1078
Number of pages6
JournalClinical Cancer Research
Volume6
Issue number3
Publication statusPublished - 2000 Mar

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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