TY - JOUR
T1 - Diversity of sex chromosomes in Sulawesian medaka fishes
AU - Ansai, Satoshi
AU - Montenegro, Javier
AU - Masengi, Kawilarang W.A.
AU - Nagano, Atsushi J.
AU - Yamahira, Kazunori
AU - Kitano, Jun
N1 - Funding Information:
We thank the Ministry of Research, Technology, and Higher Education, Republic of Indonesia (RISTEKDIKTI), and the Faculty of Fisheries and Marine Science, Sam Ratulangi University, for permission to conduct research in Sulawesi (Research Permit Numbers 394/SIP/FRP/SM/XI/2014, 397/SIP/FRP/SM/XI/2014, and 106/SIP/FRP/E5/Dit.KI/IV/2018). We also thank S. Kondo (Ryukoku University) and members of the Yamahira lab at the University of the Ryukyus for technical assistance. Cynthia Kulongowski with Edanz ( https://jp.edanz.com/ac ) edited the language of a draft of this manuscript. This work was supported by JSPS KAKENHI Grant Numbers 21 K15143 to S.A., 19 K16203 to J.M., and 21H04782 to K.Y.; and JST CREST Grant Number JPMJCR20S2 to J.K., K.Y., and S.A.
Funding Information:
We thank the Ministry of Research, Technology, and Higher Education, Republic of Indonesia (RISTEKDIKTI), and the Faculty of Fisheries and Marine Science, Sam Ratulangi University, for permission to conduct research in Sulawesi (Research Permit Numbers 394/SIP/FRP/SM/XI/2014, 397/SIP/FRP/SM/XI/2014, and 106/SIP/FRP/E5/Dit.KI/IV/2018). We also thank S. Kondo (Ryukoku University) and members of the Yamahira lab at the University of the Ryukyus for technical assistance. Cynthia Kulongowski with Edanz (https://jp.edanz.com/ac) edited the language of a draft of this manuscript. This work was supported by JSPS KAKENHI Grant Numbers 21 K15143 to S.A., 19 K16203 to J.M., and 21H04782 to K.Y.; and JST CREST Grant Number JPMJCR20S2 to J.K., K.Y., and S.A.
Publisher Copyright:
© 2022 European Society for Evolutionary Biology.
PY - 2022/12
Y1 - 2022/12
N2 - Recent genetic and genomic studies have revealed tremendous diversity in sex chromosomes across diverse taxa. Closely related species with different sex chromosomes provide us excellent opportunities to investigate the driving forces and the consequences of sex chromosome turnover. In the present study, we investigated the diversity of sex chromosomes of 13 Oryzias species from Sulawesi, Indonesia, which diversified during the last 4.86 million years. Using pooled sequencing, we found sex chromosomes in nine species that all had XY systems, with a species being possibly modified by multiple loci. Seven species (O. woworae, O. asinua, O. wolasi, O. matanensis, O. celebensis, O. hadiatyae, and O. dopingdopingensis) share linkage group (LG) 24 as sex chromosomes; however, they differed in the length and magnitude of sequence divergence between the X and Y chromosomes. The sex chromosome of O. eversi was LG4, which has not been reported as a sex chromosome in any other medaka species. In O. sarasinorum, LG16 and LG22 are associated with sex. Although LG16 was found to be sex-linked in another medaka species previously examined, the sex-determining regions did not overlap. No significant signatures for sex chromosomes were identified in the other four species (O. marmoratus, O. nigrimas, O. nebulosus, and O. orthognathus). Frequent turnovers and the great diversity of the sex chromosomes will make Sulawesian medaka species a model system for investigating the driving forces and consequences of sex chromosome turnover.
AB - Recent genetic and genomic studies have revealed tremendous diversity in sex chromosomes across diverse taxa. Closely related species with different sex chromosomes provide us excellent opportunities to investigate the driving forces and the consequences of sex chromosome turnover. In the present study, we investigated the diversity of sex chromosomes of 13 Oryzias species from Sulawesi, Indonesia, which diversified during the last 4.86 million years. Using pooled sequencing, we found sex chromosomes in nine species that all had XY systems, with a species being possibly modified by multiple loci. Seven species (O. woworae, O. asinua, O. wolasi, O. matanensis, O. celebensis, O. hadiatyae, and O. dopingdopingensis) share linkage group (LG) 24 as sex chromosomes; however, they differed in the length and magnitude of sequence divergence between the X and Y chromosomes. The sex chromosome of O. eversi was LG4, which has not been reported as a sex chromosome in any other medaka species. In O. sarasinorum, LG16 and LG22 are associated with sex. Although LG16 was found to be sex-linked in another medaka species previously examined, the sex-determining regions did not overlap. No significant signatures for sex chromosomes were identified in the other four species (O. marmoratus, O. nigrimas, O. nebulosus, and O. orthognathus). Frequent turnovers and the great diversity of the sex chromosomes will make Sulawesian medaka species a model system for investigating the driving forces and consequences of sex chromosome turnover.
KW - Adrianichthyidae
KW - Oryzias
KW - Sulawesi
KW - pool-seq
KW - sexual dimorphism
UR - http://www.scopus.com/inward/record.url?scp=85136464661&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85136464661&partnerID=8YFLogxK
U2 - 10.1111/jeb.14076
DO - 10.1111/jeb.14076
M3 - Article
C2 - 36054501
AN - SCOPUS:85136464661
SN - 1010-061X
VL - 35
SP - 1751
EP - 1764
JO - Journal of Evolutionary Biology
JF - Journal of Evolutionary Biology
IS - 12
ER -