Diversity of bradyrhizobium in non-leguminous sorghum plants: B. ottawaense isolates unique in genes for n2o reductase and lack of the type VI secretion system

Sawa Wasai-Hara, Shintaro Hara, Takashi Morikawa, Masayuki Sugawara, Hideto Takami, Junich Yoneda, Tsuyoshi Tokunaga, Kiwamu Minamisawa

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Diverse members of Bradyrhizobium diazoefficiens, B. japonicum, and B. ottawaense were isolated from the roots of field-grown sorghum plants in Fukushima, and classified into “Rhizobia” with nodulated soybeans, “Free-living diazotrophs”, and “Non-diazotrophs” by nitrogen fixation and nodulation assays. Genome analyses revealed that B. ottawaense members possessed genes for N2O reduction, but lacked those for the Type VI secretion system (T6SS). T6SS is a new bacterial weapon against microbial competitors. Since T6SS-possessing B. diazoefficiens and B. japonicum have mainly been isolated from soybean nodules in Japan, T6SS-lacking B. ottawaense members may be a cryptic lineage of soybean bradyrhizobia in Japan.

Original languageEnglish
Article numberME19102
JournalMicrobes and environments
Volume35
Issue number1
DOIs
Publication statusPublished - 2020

Keywords

  • Bradyrhizobium
  • NO reductase gene
  • Nitrogen fixation
  • Protein secretion system
  • Sorghum

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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