Diversity of bradyrhizobium in non-leguminous sorghum plants: B. ottawaense isolates unique in genes for n2o reductase and lack of the type VI secretion system

Sawa Wasai-Hara; Shintaro Hara; Takashi Morikawa; Masayuki Sugawara; Hideto Takami; Junich Yoneda; Tsuyoshi Tokunaga; Kiwamu Minamisawa

doi:10.1264/jsme2.ME19102

Diversity of bradyrhizobium in non-leguminous sorghum plants: B. ottawaense isolates unique in genes for n₂o reductase and lack of the type VI secretion system

Sawa Wasai-Hara, Shintaro Hara, Takashi Morikawa, Masayuki Sugawara, Hideto Takami, Junich Yoneda, Tsuyoshi Tokunaga, Kiwamu Minamisawa

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Diverse members of Bradyrhizobium diazoefficiens, B. japonicum, and B. ottawaense were isolated from the roots of field-grown sorghum plants in Fukushima, and classified into “Rhizobia” with nodulated soybeans, “Free-living diazotrophs”, and “Non-diazotrophs” by nitrogen fixation and nodulation assays. Genome analyses revealed that B. ottawaense members possessed genes for N₂O reduction, but lacked those for the Type VI secretion system (T6SS). T6SS is a new bacterial weapon against microbial competitors. Since T6SS-possessing B. diazoefficiens and B. japonicum have mainly been isolated from soybean nodules in Japan, T6SS-lacking B. ottawaense members may be a cryptic lineage of soybean bradyrhizobia in Japan.

Original language	English
Article number	ME19102
Journal	Microbes and environments
Volume	35
Issue number	1
DOIs	https://doi.org/10.1264/jsme2.ME19102
Publication status	Published - 2020

Keywords

Bradyrhizobium
NO reductase gene
Nitrogen fixation
Protein secretion system
Sorghum

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Soil Science
Plant Science

Access to Document

10.1264/jsme2.ME19102

Cite this

Diversity of bradyrhizobium in non-leguminous sorghum plants : B. ottawaense isolates unique in genes for n₂o reductase and lack of the type VI secretion system. / Wasai-Hara, Sawa; Hara, Shintaro; Morikawa, Takashi; Sugawara, Masayuki; Takami, Hideto; Yoneda, Junich; Tokunaga, Tsuyoshi; Minamisawa, Kiwamu.

In: Microbes and environments, Vol. 35, No. 1, ME19102, 2020.

Research output: Contribution to journal › Article › peer-review

Wasai-Hara, Sawa ; Hara, Shintaro ; Morikawa, Takashi ; Sugawara, Masayuki ; Takami, Hideto ; Yoneda, Junich ; Tokunaga, Tsuyoshi ; Minamisawa, Kiwamu. / Diversity of bradyrhizobium in non-leguminous sorghum plants : B. ottawaense isolates unique in genes for n₂o reductase and lack of the type VI secretion system. In: Microbes and environments. 2020 ; Vol. 35, No. 1.

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title = "Diversity of bradyrhizobium in non-leguminous sorghum plants: B. ottawaense isolates unique in genes for n2o reductase and lack of the type VI secretion system",

abstract = "Diverse members of Bradyrhizobium diazoefficiens, B. japonicum, and B. ottawaense were isolated from the roots of field-grown sorghum plants in Fukushima, and classified into “Rhizobia” with nodulated soybeans, “Free-living diazotrophs”, and “Non-diazotrophs” by nitrogen fixation and nodulation assays. Genome analyses revealed that B. ottawaense members possessed genes for N2O reduction, but lacked those for the Type VI secretion system (T6SS). T6SS is a new bacterial weapon against microbial competitors. Since T6SS-possessing B. diazoefficiens and B. japonicum have mainly been isolated from soybean nodules in Japan, T6SS-lacking B. ottawaense members may be a cryptic lineage of soybean bradyrhizobia in Japan.",

keywords = "Bradyrhizobium, NO reductase gene, Nitrogen fixation, Protein secretion system, Sorghum",

author = "Sawa Wasai-Hara and Shintaro Hara and Takashi Morikawa and Masayuki Sugawara and Hideto Takami and Junich Yoneda and Tsuyoshi Tokunaga and Kiwamu Minamisawa",

note = "Funding Information: This work was supported by a Grant-in-Aid for Scientific Research ? 8H021 12) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by a grant from Earthnote Co., Ltd. to K.M.. S.W. was supported by fellowships from the Division for Interdisciplinary Advanced Research and Education in Tohoku University. Funding Information: This work was supported by a Grant-in-Aid for Scientific Research (18H02112) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by a grant from Earthnote Co., Ltd. to K.M.. S.W. was supported by fellowships from the Division for Interdisciplinary Advanced Research and Education in Tohoku University. Publisher Copyright: {\textcopyright} 2020, Japanese Society of Microbial Ecology. All rights reserved.",

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AU - Hara, Shintaro

AU - Morikawa, Takashi

AU - Sugawara, Masayuki

AU - Takami, Hideto

AU - Yoneda, Junich

AU - Tokunaga, Tsuyoshi

AU - Minamisawa, Kiwamu

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PY - 2020

Y1 - 2020

N2 - Diverse members of Bradyrhizobium diazoefficiens, B. japonicum, and B. ottawaense were isolated from the roots of field-grown sorghum plants in Fukushima, and classified into “Rhizobia” with nodulated soybeans, “Free-living diazotrophs”, and “Non-diazotrophs” by nitrogen fixation and nodulation assays. Genome analyses revealed that B. ottawaense members possessed genes for N2O reduction, but lacked those for the Type VI secretion system (T6SS). T6SS is a new bacterial weapon against microbial competitors. Since T6SS-possessing B. diazoefficiens and B. japonicum have mainly been isolated from soybean nodules in Japan, T6SS-lacking B. ottawaense members may be a cryptic lineage of soybean bradyrhizobia in Japan.

AB - Diverse members of Bradyrhizobium diazoefficiens, B. japonicum, and B. ottawaense were isolated from the roots of field-grown sorghum plants in Fukushima, and classified into “Rhizobia” with nodulated soybeans, “Free-living diazotrophs”, and “Non-diazotrophs” by nitrogen fixation and nodulation assays. Genome analyses revealed that B. ottawaense members possessed genes for N2O reduction, but lacked those for the Type VI secretion system (T6SS). T6SS is a new bacterial weapon against microbial competitors. Since T6SS-possessing B. diazoefficiens and B. japonicum have mainly been isolated from soybean nodules in Japan, T6SS-lacking B. ottawaense members may be a cryptic lineage of soybean bradyrhizobia in Japan.

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KW - Protein secretion system

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