TY - JOUR
T1 - Diversity of bradyrhizobium in non-leguminous sorghum plants
T2 - B. ottawaense isolates unique in genes for n2o reductase and lack of the type VI secretion system
AU - Wasai-Hara, Sawa
AU - Hara, Shintaro
AU - Morikawa, Takashi
AU - Sugawara, Masayuki
AU - Takami, Hideto
AU - Yoneda, Junich
AU - Tokunaga, Tsuyoshi
AU - Minamisawa, Kiwamu
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research ? 8H021 12) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by a grant from Earthnote Co., Ltd. to K.M.. S.W. was supported by fellowships from the Division for Interdisciplinary Advanced Research and Education in Tohoku University.
Publisher Copyright:
© 2020, Japanese Society of Microbial Ecology. All rights reserved.
PY - 2020
Y1 - 2020
N2 - Diverse members of Bradyrhizobium diazoefficiens, B. japonicum, and B. ottawaense were isolated from the roots of field-grown sorghum plants in Fukushima, and classified into “Rhizobia” with nodulated soybeans, “Free-living diazotrophs”, and “Non-diazotrophs” by nitrogen fixation and nodulation assays. Genome analyses revealed that B. ottawaense members possessed genes for N2O reduction, but lacked those for the Type VI secretion system (T6SS). T6SS is a new bacterial weapon against microbial competitors. Since T6SS-possessing B. diazoefficiens and B. japonicum have mainly been isolated from soybean nodules in Japan, T6SS-lacking B. ottawaense members may be a cryptic lineage of soybean bradyrhizobia in Japan.
AB - Diverse members of Bradyrhizobium diazoefficiens, B. japonicum, and B. ottawaense were isolated from the roots of field-grown sorghum plants in Fukushima, and classified into “Rhizobia” with nodulated soybeans, “Free-living diazotrophs”, and “Non-diazotrophs” by nitrogen fixation and nodulation assays. Genome analyses revealed that B. ottawaense members possessed genes for N2O reduction, but lacked those for the Type VI secretion system (T6SS). T6SS is a new bacterial weapon against microbial competitors. Since T6SS-possessing B. diazoefficiens and B. japonicum have mainly been isolated from soybean nodules in Japan, T6SS-lacking B. ottawaense members may be a cryptic lineage of soybean bradyrhizobia in Japan.
KW - Bradyrhizobium
KW - NO reductase gene
KW - Nitrogen fixation
KW - Protein secretion system
KW - Sorghum
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U2 - 10.1264/jsme2.ME19102
DO - 10.1264/jsme2.ME19102
M3 - Article
C2 - 31932539
AN - SCOPUS:85077786428
VL - 35
JO - Microbes and Environments
JF - Microbes and Environments
SN - 1342-6311
IS - 1
M1 - ME19102
ER -