TY - JOUR
T1 - Diversity and field site variation of indigenous populations of soybean bradyrhizobia in Japan by fingerprints with repeated sequences RSα and RSβ
AU - Minamisawa, Kiwamu
AU - Nakatsuka, Yoko
AU - Isawa, Tsuyoshi
N1 - Funding Information:
This work was supported in part by grants from the Ministry of Education, Science and Culture of Japan (Nos. 02455007 and 07660077), and the Joint Research Program of the Institute of Genetic Ecology, Tohoku University (Nos. 942206 and 953009). The authors gratefully acknowledge Dr. T. Asami, Dr. M. Kubota (Ibaraki University) and Dr. T. Hattori (Tohoku University) for their continuing interest.
PY - 1999/6
Y1 - 1999/6
N2 - Two hundred and thirteen isolates of soybean bradyrhizobia indigenous to six field sites in Japan were characterized using hybridization probes RSα, RSβ, nifDK and hupSL from Bradyrhizobium japonicum and indole-3-acetic acid production to clarify diversity and endemism of their population structures. Significant diversities and site-dependent variations were observed in terms of Bradyrhizobium species, hup genotype and fingerprints with repeated sequences RSα and RSβ. The fingerprints at one site with no history of soybean cultivation were less diverse than those at other sites. Even within the populations of B. japonicum hup- isolates, which were commonly indigenous to the six field sites, several RSα copies clustering around nif genes were highly conserved. The results suggest that soybean bradyrhizobia may be diversified in individual fields as associated with host plants and local soil conditions.
AB - Two hundred and thirteen isolates of soybean bradyrhizobia indigenous to six field sites in Japan were characterized using hybridization probes RSα, RSβ, nifDK and hupSL from Bradyrhizobium japonicum and indole-3-acetic acid production to clarify diversity and endemism of their population structures. Significant diversities and site-dependent variations were observed in terms of Bradyrhizobium species, hup genotype and fingerprints with repeated sequences RSα and RSβ. The fingerprints at one site with no history of soybean cultivation were less diverse than those at other sites. Even within the populations of B. japonicum hup- isolates, which were commonly indigenous to the six field sites, several RSα copies clustering around nif genes were highly conserved. The results suggest that soybean bradyrhizobia may be diversified in individual fields as associated with host plants and local soil conditions.
KW - Bradyrhizobium elkanii
KW - Bradyrhizobium japonicum
KW - Diversity
KW - Repeated sequence
KW - Soybean
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U2 - 10.1016/S0168-6496(99)00009-4
DO - 10.1016/S0168-6496(99)00009-4
M3 - Article
AN - SCOPUS:0032784377
VL - 29
SP - 171
EP - 178
JO - FEMS Microbiology Ecology
JF - FEMS Microbiology Ecology
SN - 0168-6496
IS - 2
ER -