Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): Application to serial protein crystallography using an X-ray free-electron laser

Kensuke Tono; Eriko Nango; Michihiro Sugahara; Changyong Song; Jaehyun Park; Tomoyuki Tanaka; Rie Tanaka; Yasumasa Joti; Takashi Kameshima; Shun Ono; Takaki Hatsui; Eiichi Mizohata; Mamoru Suzuki; Tatsuro Shimamura; Yoshiki Tanaka; So Iwata; Makina Yabashi

doi:10.1107/S1600577515004464

Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): Application to serial protein crystallography using an X-ray free-electron laser

Kensuke Tono, Eriko Nango, Michihiro Sugahara, Changyong Song, Jaehyun Park, Tomoyuki Tanaka, Rie Tanaka, Yasumasa Joti, Takashi Kameshima, Shun Ono, Takaki Hatsui, Eiichi Mizohata, Mamoru Suzuki, Tatsuro Shimamura, Yoshiki Tanaka, So Iwata, Makina Yabashi

Research output: Contribution to journal › Article › peer-review

61 Citations (Scopus)

Abstract

An experimental system for serial femtosecond crystallography using an X-ray free-electron laser (XFEL) has been developed. It basically consists of a sample chamber, fluid injectors and a two-dimensional detector. The chamber and the injectors are operated under helium atmosphere at 1atm. The ambient pressure operation facilitates applications to fluid samples. Three kinds of injectors are employed to feed randomly oriented crystals in aqueous solution or highly viscous fluid. Experiments on lysozyme crystals were performed by using the 10keV XFEL of the SPring-8 Angstrom Compact free-electron LAser (SACLA). The structure of model protein lysozyme from 1μm crystals at a resolution of 2.4Å was obtained.

Original language	English
Pages (from-to)	532-537
Number of pages	6
Journal	Journal of Synchrotron Radiation
Volume	22
DOIs	https://doi.org/10.1107/S1600577515004464
Publication status	Published - 2015 May 1
Externally published	Yes

Keywords

XFEL Special issue on X-ray Free-Electron Lasers
serial femtosecond crystallography

ASJC Scopus subject areas

Radiation
Nuclear and High Energy Physics
Instrumentation

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10.1107/S1600577515004464

Cite this

Tono, K., Nango, E., Sugahara, M., Song, C., Park, J., Tanaka, T., Tanaka, R., Joti, Y., Kameshima, T., Ono, S., Hatsui, T., Mizohata, E., Suzuki, M., Shimamura, T., Tanaka, Y., Iwata, S., & Yabashi, M. (2015). Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): Application to serial protein crystallography using an X-ray free-electron laser. Journal of Synchrotron Radiation, 22, 532-537. https://doi.org/10.1107/S1600577515004464

Tono, K, Nango, E, Sugahara, M, Song, C, Park, J, Tanaka, T, Tanaka, R, Joti, Y, Kameshima, T, Ono, S, Hatsui, T, Mizohata, E, Suzuki, M, Shimamura, T, Tanaka, Y, Iwata, S & Yabashi, M 2015, 'Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): Application to serial protein crystallography using an X-ray free-electron laser', Journal of Synchrotron Radiation, vol. 22, pp. 532-537. https://doi.org/10.1107/S1600577515004464

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abstract = "An experimental system for serial femtosecond crystallography using an X-ray free-electron laser (XFEL) has been developed. It basically consists of a sample chamber, fluid injectors and a two-dimensional detector. The chamber and the injectors are operated under helium atmosphere at 1atm. The ambient pressure operation facilitates applications to fluid samples. Three kinds of injectors are employed to feed randomly oriented crystals in aqueous solution or highly viscous fluid. Experiments on lysozyme crystals were performed by using the 10keV XFEL of the SPring-8 Angstrom Compact free-electron LAser (SACLA). The structure of model protein lysozyme from 1μm crystals at a resolution of 2.4{\AA} was obtained.",

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author = "Kensuke Tono and Eriko Nango and Michihiro Sugahara and Changyong Song and Jaehyun Park and Tomoyuki Tanaka and Rie Tanaka and Yasumasa Joti and Takashi Kameshima and Shun Ono and Takaki Hatsui and Eiichi Mizohata and Mamoru Suzuki and Tatsuro Shimamura and Yoshiki Tanaka and So Iwata and Makina Yabashi",

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AU - Joti, Yasumasa

AU - Kameshima, Takashi

AU - Ono, Shun

AU - Hatsui, Takaki

AU - Mizohata, Eiichi

AU - Suzuki, Mamoru

AU - Shimamura, Tatsuro

AU - Tanaka, Yoshiki

AU - Iwata, So

AU - Yabashi, Makina

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Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): Application to serial protein crystallography using an X-ray free-electron laser

Abstract

Keywords

ASJC Scopus subject areas

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