Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): Application to serial protein crystallography using an X-ray free-electron laser

Kensuke Tono, Eriko Nango, Michihiro Sugahara, Changyong Song, Jaehyun Park, Tomoyuki Tanaka, Rie Tanaka, Yasumasa Joti, Takashi Kameshima, Shun Ono, Takaki Hatsui, Eiichi Mizohata, Mamoru Suzuki, Tatsuro Shimamura, Yoshiki Tanaka, So Iwata, Makina Yabashi

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

An experimental system for serial femtosecond crystallography using an X-ray free-electron laser (XFEL) has been developed. It basically consists of a sample chamber, fluid injectors and a two-dimensional detector. The chamber and the injectors are operated under helium atmosphere at 1atm. The ambient pressure operation facilitates applications to fluid samples. Three kinds of injectors are employed to feed randomly oriented crystals in aqueous solution or highly viscous fluid. Experiments on lysozyme crystals were performed by using the 10keV XFEL of the SPring-8 Angstrom Compact free-electron LAser (SACLA). The structure of model protein lysozyme from 1μm crystals at a resolution of 2.4Å was obtained.

Original languageEnglish
Pages (from-to)532-537
Number of pages6
JournalJournal of Synchrotron Radiation
Volume22
DOIs
Publication statusPublished - 2015 May 1
Externally publishedYes

Keywords

  • serial femtosecond crystallography
  • XFEL Special issue on X-ray Free-Electron Lasers

ASJC Scopus subject areas

  • Radiation
  • Nuclear and High Energy Physics
  • Instrumentation

Fingerprint Dive into the research topics of 'Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): Application to serial protein crystallography using an X-ray free-electron laser'. Together they form a unique fingerprint.

  • Cite this

    Tono, K., Nango, E., Sugahara, M., Song, C., Park, J., Tanaka, T., Tanaka, R., Joti, Y., Kameshima, T., Ono, S., Hatsui, T., Mizohata, E., Suzuki, M., Shimamura, T., Tanaka, Y., Iwata, S., & Yabashi, M. (2015). Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): Application to serial protein crystallography using an X-ray free-electron laser. Journal of Synchrotron Radiation, 22, 532-537. https://doi.org/10.1107/S1600577515004464