Divergent effects of estrogen and nicotine on Rho-kinase expression in human coronary vascular smooth muscle cells

Junko Hiroki, Hiroaki Shimokawa, Yasushi Mukai, Toshihiro Ichiki, Akira Takeshita

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

Recent studies have demonstrated that up-regulated Rho-kinase plays an important role in the pathogenesis of coronary arteriosclerosis and vasospasm. We have shown that inflammatory stimuli, such as angiotensin II and interleukin-1β, up-regulate Rho-kinase expression and activity in human coronary vascular smooth muscle cells, for which intracellular signal transduction mediated by protein kinase C and NF-κB is involved. Here, we show that estrogen down-regulates while nicotine up-regulates Rho-kinase and that nicotine counteracts the inhibitory effect of estrogen on angiotensin II-induced Rho-kinase expression. Furthermore, we demonstrated that the intracellular signal transduction of the inhibitory effect of estrogen is mediated by an estrogen receptor. These results demonstrate that inflammatory stimuli up-regulate Rho-kinase, for which estrogen (mediated by an estrogen receptor) and nicotine exert divergent inhibitory and stimulatory effects on the Rho-kinase expression, respectively, and may explain in part why the incidence of arteriosclerotic and vasospastic disorders is increased in postmenopausal women and smokers.

Original languageEnglish
Pages (from-to)154-159
Number of pages6
JournalBiochemical and biophysical research communications
Volume326
Issue number1
DOIs
Publication statusPublished - 2004 Dec 31
Externally publishedYes

Keywords

  • Coronary arteriosclerosis
  • Estrogen
  • Estrogen receptor
  • Nicotine
  • Rho-kinase
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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