Distribution of type I collagen, type II collagen and PNA binding glycoconjugates during chondrogenesis of three distinct embryonic cartilages

Yasuyuki Sasano, Itaru Mizoguchi, Manabu Kagayama, Lillian Shum, Pablo Bringas, Harold C. Slavkin

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Previous studies of chondrogenesis have been focused on limb bud cartilage, whereas little is known about chondrogenic processes of other cartilages with different developmental fates. We hypothesize that cartilages with various developmental fates might show identical characteristics of chondrogenesis. The chondrogenic processes in the nasal septum, the mandible, and the limb bud of the mouse were examined by means of PNA-binding glycoconjugate, and types I and II collagen expression. Swiss-Webster mouse embryos of 11 days (E11) to 14 days (E14) gestation were fixed and processed for imniuno- and lectin histochemistry. The blastema of mesenchymal cell aggregates stained positively with anti-type I collagen, but very weakly with anti-type II collagen in all three models at E12, whereas PNA bound to the blastema in the limb bud but not in nasal septum or mandible. Types I and II collagens coexisted in cartilages at E13. Type II collagen was predominant in E14; type I collagen was confined to the peripheral region. The synchronized transitional expression of the collagen phenotypes in all three embryonic cartilages may be systemically regulated. The presence or absence of the PNA-binding glycoconjugates may be involved in characterizing the nature of the cartilages.

Original languageEnglish
Pages (from-to)205-213
Number of pages9
JournalAnatomy and Embryology
Volume186
Issue number3
DOIs
Publication statusPublished - 1992 Aug 1

Keywords

  • Chondrogenesis
  • Collagen
  • Limb bud
  • Meckel's cartilage
  • Nasal septum
  • PNA

ASJC Scopus subject areas

  • Anatomy
  • Embryology
  • Developmental Biology
  • Cell Biology

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