Distribution of transient receptor potential melastatin-8-containing nerve fibers in rat oral and craniofacial structures

Takehiro Yajima, Tadasu Sato, Hiroshi Hosokawa, Teruyoshi Kondo, Masahiro Saito, Hidetoshi Shimauchi, Hiroyuki Ichikawa

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


The transient receptor potential melastatin-8 (TRPM8) is a cold and menthol receptor located in the sensory ganglia. Immunohistochemistry for TRPM8 was performed on oral and craniofacial structures of the rat. TRPM8-immunoreactive (-IR) nerve fibers were detected in the oral mucous membrane. In the gingiva, TRPM8-IR nerve fibers were abundant beneath and within crestal and outer epithelia. Such nerve fibers were also common beneath and within taste buds in the incisive papilla. In addition, TRPM8-immunoreactivity was expressed by some taste bud cells in the papilla. Lips, periodontal ligaments and salivary glands as well as masticatory muscles and temporomandibular joints were mostly devoid of TRPM8-IR nerve fibers. A double immunofluorescence study indicated different distribution patterns of nerve fibers containing TRPM8 and calcitonin gene-related peptide in oral and craniofacial tissues. Retrograde tracing method also indicated that TRPM8-IR nerve fibers in the gingiva and incisive papilla originate from small sensory neurons in the trigeminal ganglion. TRPM8 may be associated with cool, cold nociceptive (<around 25 °C) and chemoreceptive transmission in the oral mucosa.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalAnnals of Anatomy
Publication statusPublished - 2015 Sep 1


  • Gingiva
  • Immunohistochemistry
  • Incisive papilla
  • Rat
  • Transient receptor potential melastatin-8
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology


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