Distribution of tetrodotoxin, saxitoxin, and their analogs among tissues of the puffer fish Fugu pardalis

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Abstract

The anatomical distribution of tetrodotoxin (TTX), saxitoxin (STX) and their analogs (TTXs, STXs) in three female and three male specimens of the marine puffer fish Fugu pardalis from Miyagi Prefecture, 2005, Japan, were studied. 5-DeoxyTTX, 11-deoxyTTX, and 5,6,11-trideoxyTTX were quantified by liquid chromatography/mass spectrometry (LC/MS) for the first time, and other TTXs and STXs were determined by liquid chromatography-fluorescent detection (LC-FLD). As a result, 5,6,11-trideoxyTTX was found to be the major TTX analog in all tissues tested, whereas 5-deoxyTTX and 11-deoxyTTX were minor components. Especially, in female (n = 3), the ratios of 5,6,11-trideoxyTTX to total of all TTX analogs (mole/mole) in ovaries (mean±SD, 0.42±0.055) were significantly larger than those in livers (0.17±0.025) (P < 0.05). In contrary, the ratios of 4,9-anhydroTTX to total of all TTX analogs in livers (0.27±0.047) were significantly larger than those in ovaries (0.073±0.040) (P < 0.01). The ratios of TTX to total of all TTX analogs were not significantly different between ovaries (0.47±0.078) and livers (0.55±0.067). In male (n = 3), all these ratios were not significantly different between livers and testis. 4-S-CysteinylTTX was detected in liver, spleen, gall, and intestine in 1-6 mole% of total of all TTX analogs, supporting our previous hypothesis that 4-S-cysteinylTTX is a metabolite of TTX.

Original languageEnglish
Pages (from-to)980-987
Number of pages8
JournalToxicon
Volume48
Issue number8
DOIs
Publication statusPublished - 2006 Dec 15

Keywords

  • LC/MS
  • Puffer fish
  • Saxitoxin
  • Tetrodotoxin

ASJC Scopus subject areas

  • Toxicology

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