TY - JOUR
T1 - Distribution of Retinoids Applied Exogenously to Chick Limb Buds
T2 - an Autoradiographic Analysis: Retinoic Acid/Limb Bud/Pattern Formation/Autoradiograph
AU - Tamura, Koji
AU - Hashimoto, Yuichi
AU - Shudo, Koichi
AU - Ide, Hiroyuki
PY - 1993/10
Y1 - 1993/10
N2 - An autoradiographic analysis was undertaken to examine the localization of retinoids applied exogenously to chick limb buds. Ion‐exchange beads (AGI‐X2) containing a tritium‐labeled synthetic retinoid, Am80, were implanted to various regions of chick wing buds. This synthetic retinoid is known to induce a duplicated limb pattern as retinoic acid (RA) does. One to 24 hours after the application, wing buds were fixed, sectioned, and prepared for autoradiography. Heavy labeling was observed in the peripheral region of the wing mesoderm, but no gradient along the antero‐posterior axis was found. These results suggest that the peripheral region of the limb bud may be important for the morphogenetic function of RA. Tissue‐bound retinoids may not form an antero‐posterior concentration gradient when retinoids are added to the anterior margin of the chick limb bud.
AB - An autoradiographic analysis was undertaken to examine the localization of retinoids applied exogenously to chick limb buds. Ion‐exchange beads (AGI‐X2) containing a tritium‐labeled synthetic retinoid, Am80, were implanted to various regions of chick wing buds. This synthetic retinoid is known to induce a duplicated limb pattern as retinoic acid (RA) does. One to 24 hours after the application, wing buds were fixed, sectioned, and prepared for autoradiography. Heavy labeling was observed in the peripheral region of the wing mesoderm, but no gradient along the antero‐posterior axis was found. These results suggest that the peripheral region of the limb bud may be important for the morphogenetic function of RA. Tissue‐bound retinoids may not form an antero‐posterior concentration gradient when retinoids are added to the anterior margin of the chick limb bud.
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U2 - 10.1111/j.1440-169X.1993.00593.x
DO - 10.1111/j.1440-169X.1993.00593.x
M3 - Article
AN - SCOPUS:0027495980
VL - 35
SP - 593
EP - 599
JO - Development Growth and Differentiation
JF - Development Growth and Differentiation
SN - 0012-1592
IS - 5
ER -