Distribution of protein disulfide isomerase during maturation of pig oocytes

Yumi Ohashi, Yumi Hoshino, Kentaro Tanemura, Eimei Sato

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Oocyte maturation in mammals is characterized by a dramatic reorganization of the endoplasmic reticulum (ER). In mice, the ER forms accumulations in the germinal vesicle (GV) stage and distinctive cortical clusters in metaphase II (MII) of the oocyte. Multiple evidence suggests that this ER distribution is important in preparing the oocyte for Ca2+ oscillations, which trigger oocyte activation at fertilization. In this study, we investigated the time course and illustrated the possible functional role of ER distribution during maturation of porcine oocytes by immunostaining with protein disulfide isomerase (PDI). PDI forms clusters in the cytoplasm of oocytes. After immunostaining, PDI clusters were identified throughout the cytoplasm from the GV to metaphase I (MI) stage; however, at the MII stage, the PDI formed large clusters (1-2μm) in the animal pole around the first polar body. PDI distribution was prevented by bacitracin, a PDI inhibitor. Our experiments indicated that, during porcine oocyte maturation, PDI undergoes a dramatic reorganization. This characteristic distribution is different from that in the mouse oocyte. Moreover, our study suggested that formation of PDI clusters in the animal pole is a specific characteristic of matured porcine oocytes.

Original languageEnglish
Pages (from-to)15-22
Number of pages8
JournalAnimal Science Journal
Volume84
Issue number1
DOIs
Publication statusPublished - 2013 Jan

Keywords

  • Endoplasmic reticulum
  • Oocyte maturation
  • Porcine
  • Protein disulfide isomerase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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