Distribution of multiple peptidoglycan recognition proteins in the tissues of Pacific oyster, Crassostrea gigas

Naoki Itoh, Keisuke G. Takahashi

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Peptidoglycan recognition proteins (PGRPs) are pattern recognition receptors that specifically bind to peptidoglycans, a major component of bacterial cell wall. Generally, PGRPs are responsible for recognition of bacterial invasion in invertebrates. Full length cDNAs of PGRP, designated as CgPGRP-S1S, -S1L, -S2 and -S3, were identified from the Pacific oyster, Crassostrea gigas. Homology and domain searches classified these CgPGRPs as short-type PGRPs for extracellular PGN recognition. Amidase activity was predicted in all CgPGRPs, and defensin-like domains were found in CgPGRP-S1S and -S1L, suggesting that they may also function as antimicrobial proteins. Although phylogenetic analysis indicated that CgPGRPs are closely related to each other, they showed different tissue expression patterns; CgPGRP-S1S in the mantle and the gill, -S1L in the mantle, -S2 in the hemocytes and -S3 in the digestive diverticula. The CgPGRPs seem to survey bacterial invasion in their corresponding expression tissues. This is the first report of the possibility that bivalve mollusks have PGN recognition systems as suggested by the identification of multiple PGRPs distributed in various tissues.

Original languageEnglish
Pages (from-to)409-417
Number of pages9
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume150
Issue number4
DOIs
Publication statusPublished - 2008 Aug

Keywords

  • Bivalves
  • Crassostrea gigas
  • Gene expression
  • Innate immunity
  • Pacific oyster
  • Pattern recognition receptors (PRRs)
  • Peptidoglycan recognition proteins (PGRPs)
  • Peptidoglycans (PGN)

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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