Distribution of glycoconjugates localized by peanut and maclura pomifera agglutinins during mouse molar root development

Y. Sasano, Y. Kaji, M. Nakamura, K. Kindaichi, H. C. Slavkin, M. Kagayama

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Glycolipids, glycoproteins, glycosaminoglycans and sialoglycoproteins have all been implicated in a number of developmentally significant processes related to complex interactions between cell surfaces and the extracellular matrix. The present study was designed to localize glycoconjugates recognizcd by peanut agglutinin (PNA) and Maclura pomifera (MPA) lectins during mouse molar root development. Postnatal ICR mice at 10. 15, 21. 28 and 42 days were used. Lower jaws were dissected, fixed in 4% paraformaldehyde, decalcified in 5% EDTA and embedded in paraffin. Serial sections were made and stained with FITC-conjugated PNA or MPA. (β-Lactose was used as an inhibitory sugar for PNA, and α-D-melibiose for MPA. PNA specifically stained Hertwig’s epithelial root sheath (HERS), whereas MPA stained a number of tissues. The outermost layer of root dentin, forming cellular cementum, alveolar bone and HERS showed positive reactions with MPA. Glycoconjugates localized by the lectins may be functionally related to molecules which contribute to root formation and cementogenesis.

Original languageEnglish
Pages (from-to)149-155
Number of pages7
JournalCells Tissues Organs
Volume145
Issue number2
DOIs
Publication statusPublished - 1992 Jan 1

Keywords

  • Cementogenesis
  • Hertwig’s epithelial root sheath
  • Intermediate cementum
  • Lectins
  • Mouse
  • Root formation
  • Tooth

ASJC Scopus subject areas

  • Anatomy
  • Histology

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