Distribution of Cryptosporidium species isolated from diarrhoeic calves in Japan

Mohammad Hazzaz Bin Kabir, Megumi Itoh, Ayman Ahmed Shehata, Hironori Bando, Yasuhiro Fukuda, Fumi Murakoshi, Atsushi Fujikura, Hiroaki Okawa, Takuto Endo, Akira Goto, Masayuki Kachi, Toshie Nakayama, Yuto Kano, Shoko Oishi, Konosuke Otomaru, Mohamed Ibrahim Essa, Kei Kazama, Xuenan Xuan, Kentaro Kato

Research output: Contribution to journalArticlepeer-review

Abstract

Cryptosporidium spp. are enteric protozoan parasites that infect a wide range of hosts including humans, and domestic and wild animals. The aim of this study was to molecularly characterize the Cryptosporidium spp. found in calf faeces in Japan. A total of 80 pre-weaned beef and dairy calves' diarrhoeic faecal specimens were collected from nine different prefectures in Japan. A nested polymerase chain reaction targeting the small subunit 18S rRNA and GP60 genes were used to detect the Cryptosporidium genotypes and subtypes. 83.8% (67 out of 80) of the specimens were positive for Cryptosporidium spp.; Cryptosporidium was found in both beef and dairy calves. Cryptosporidium parvum was the predominant species, detected in 77.5% (31/40) of beef calves and 80% (32/40) of dairy calves. Cryptosporidium bovis was also detected, 5.0% (2/40) of dairy calves, and C. ryanae was also found 2.5% (1/40) of dairy calves. One mixed-species infection, 2.5% (1/40) was detected in a beef calf having C. parvum, and C. ryanae. We detected the most common subtype of C. parvum (i.e., IIaA15G2R1), as well as other subtypes (i.e., IIaA14G3R1, IIaA14G2R1, and IIaA13G1R1) that have not previously been detected in calves in Japan. Our results demonstrate the widespread diversity of Cryptosporidium infection in calves in Japan.

Original languageEnglish
Article number102153
JournalParasitology International
Volume78
DOIs
Publication statusPublished - 2020 Oct

Keywords

  • C. parvum
  • Cryptosporidium
  • Japan
  • PCR-based sequencing
  • Phylogenetic analyses
  • Pre-weaned calves

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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