Distribution of Ca2+ channels on cochlear outer hair cells revealed by fluorescent dihydropyridines

Takeshi Oshima, Katsuhisa Ikeda, Masayuki Furukawa, Narihisa Ueda, Hideaki Suzuki, Tomonori Takasaka

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Physiological evidence has shown that cochlear outer hair cells (OHC) possess L-type voltage-dependent Ca2+ channels through which Ca2+ enters the OHC during depolarization. Their subcellular distribution has, however, remained unclear. In this study, the distribution of L-type Ca2+ channels on the basolateral plasma membrane of OHC has been demonstrated by the use of a laser scanning confocal microscope (LSCM) and a fluorescent probe DMBODIPY- DHP. The fluorescent staining pattern on the baselateral wall is nonuniform, suggesting a heterogeneous distribution of the channels in the plasma membrane. Direct imaging of intracellular Ca2+ visualized in real time by means of the LSCM and the fluorescent Ca2+ probe due 3 revealed temporal and spatial integration of Ca2+ movements and Ca2+ channel distribution. Exposure to high-K+ solution induced heterogeneity in the subcellular increase in the intracellular Ca2+ concentration. These results suggest that the heterogeneous distribution of L-type Ca2+ channels on the basolateral membrane might induce heterogeneous intracellular Ca2+ distribution during electrical activity in the OHC.

Original languageEnglish
Pages (from-to)C944-C949
JournalAmerican Journal of Physiology - Cell Physiology
Volume271
Issue number3 40-3
DOIs
Publication statusPublished - 1996 Sep

Keywords

  • L-type voltage-dependent calcium ion channels
  • inner ear
  • laser scanning confocal microscope

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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