TY - JOUR
T1 - Distribution of ammonia assimilating bacteria in the composting process
AU - Sasaki, Hiraku
AU - Maruyama, Gen
AU - Suzuki, Hanatsu
AU - Nonaka, Jun
AU - Sato, Masaaki
AU - Sasaki, Takako
AU - Ohta, Minoru
AU - Nakai, Yutaka
N1 - Funding Information:
We are grateful to Iwate Agricultural Research Center for supplying the mature compost. We also thank H. Chubachi and F. Yusa of the Experimental Farm of Graduate School of Agricultural Science, To-hoku University for technical assistance. This study was supported in part by a grant from the Livestock Technology Association, Japan. A part of this research was carried out with the aid of Science Research Subsidies on Waste Treatment and Management, the Ministry of Environment.
PY - 2004
Y1 - 2004
N2 - Ammonia assimilating bacteria were isolated from composting processes and their abilities to assimilate ammonia were evaluated. In the prefermentation stage of the composting, 104.7 and 103.7 (CFU/g) of ammonia assimilating bacteria were detected on the medium which contained ammonia as a sole nitrogen, at 37°C and 55°C, respectively. They were 104.6 and 103.2 in the end of the primary fermentation, and 105.1 and 103.2 in the end of the secondary fermentation, respectively. When isolates were purely cultivated in sterilized compost extract medium, many of them consumed ammonia not by nitrification but by assimilation. They still assimilated ammonia even in nonsterilized compost extract medium, i.e. mixed culture with the live microbial flora of the compost. However, isolates which showed high ammonia assimilating ability in the pure culture did not always show high abilities in nonsterilized compost extract media. Isolates which showed high ammonia assimilating ability in the nonsterilized medium were identified by analysis of 16S ribosomal DNA. Dominant species of ammonia assimilating microorganisms varied as composting proceeded.
AB - Ammonia assimilating bacteria were isolated from composting processes and their abilities to assimilate ammonia were evaluated. In the prefermentation stage of the composting, 104.7 and 103.7 (CFU/g) of ammonia assimilating bacteria were detected on the medium which contained ammonia as a sole nitrogen, at 37°C and 55°C, respectively. They were 104.6 and 103.2 in the end of the primary fermentation, and 105.1 and 103.2 in the end of the secondary fermentation, respectively. When isolates were purely cultivated in sterilized compost extract medium, many of them consumed ammonia not by nitrification but by assimilation. They still assimilated ammonia even in nonsterilized compost extract medium, i.e. mixed culture with the live microbial flora of the compost. However, isolates which showed high ammonia assimilating ability in the pure culture did not always show high abilities in nonsterilized compost extract media. Isolates which showed high ammonia assimilating ability in the nonsterilized medium were identified by analysis of 16S ribosomal DNA. Dominant species of ammonia assimilating microorganisms varied as composting proceeded.
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U2 - 10.1080/1065657X.2004.10702168
DO - 10.1080/1065657X.2004.10702168
M3 - Article
AN - SCOPUS:3142716047
VL - 12
SP - 108
EP - 113
JO - Compost Science and Utilization
JF - Compost Science and Utilization
SN - 1065-657X
IS - 2
ER -