Distribution of actin-filament bundles in myoid cells, Sertoli cells, and tunica albuginea of rat and mouse testes

Mamiko Maekawa, Toshio Nagano, Kyoko Kamimura, Tohru Murakami, Harunori Ishikawa, Mari Dezawa

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Frozen sections of the rat and mouse testes were stained with either FITC-phalloidin or NBD-phallacidin and viewed with conventional fluorescence and confocal laser microscopes in order to demonstrate the arrangment of actin-filament bundles in myoid cells, Sertoli cells and tunica albuginea. Myoid cells are rich in actin-filament bundles crossing at right angles. These bundles running in different directions can also be visualized by means of electron microscopy. Nerve fibers occur in the vicinity of myoid cells, suggesting a neural control of the cell. At Sertoli cell junctions actin filaments occur at the circumference of the cell, where they show a honeycomb pattern. The ratio of the number of Sertoli cells per myoid cell can be calculated by means of confocal microscopy; this technique may provide a new parameter for determining spermatogenic activity. In the tunica albuginea of the juvenile mouse testis, actin filaments are arranged in an alternate fashion.

Original languageEnglish
Pages (from-to)295-300
Number of pages6
JournalCell and Tissue Research
Volume266
Issue number2
DOIs
Publication statusPublished - 1991 Nov 1
Externally publishedYes

Keywords

  • Actin filaments
  • Confocal laser microscopy
  • Mouse (BALB)
  • Myoid cells
  • Nerve fibers
  • Rat (Wistar)
  • Sertoli cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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