Distribution of γ-hexachlorocyclohexane-degrading genes on three replicons in Sphingobium japonicum UT26

Yuji Nagata, Mayuko Kamakura, Ryo Endo, Ryo Miyazaki, Yoshiyuki Ohtsubo, Masataka Tsuda

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Sphingobium japonicum (formerly Sphingomonas paucimobilis) UT26 utilizes the important insecticide γ-hexachlorocyclohexane as a sole source of carbon and energy. In previous studies, we isolated and characterized six structural genes (linA to linF) and one regulatory gene (linR) of UT26 for the degradation of γ-hexachlorocyclohexane to β-ketoadipate. Our analysis in this study indicated that the UT26 genome consists of three large circular replicons of 3.6 Mb, 670 kb, and 185 kb. The 3.6 Mb and the 670 kb replicons had one and two copies, respectively, of the 16S ribosomal RNA gene, and these replicons were designated as chromosomes (Chr) I and II, respectively. Chr I was indicated to be a main chromosome carrying the dnaA gene. The first three lin genes, linA to linC, for conversion of γ-hexachlorocyclohexane to 2,5-dichlorohydroquinone, were dispersed on Chr I. The 185 kb plasmid, pCHQ1, carried the linRED operon for the conversion of 2,5-dichlorohydroquinone to maleylacetate and was conjugatively transferred to another sphingomonad strain. The linF gene encoding maleylacetate reductase was located on Chr II. These results indicated that the genes for the complete γ-hexachlorocyclohexane degradation are dispersed on the three large replicons of UT26.

Original languageEnglish
Pages (from-to)112-118
Number of pages7
JournalFEMS Microbiology Letters
Volume256
Issue number1
DOIs
Publication statusPublished - 2006 Mar

Keywords

  • Dehalogenase
  • Genome
  • Sphingomonad
  • γ-hexachlorocyclohexane

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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