Distribution and molecular evolution of rhamnose-binding lectins in salmonidae: Isolation and characterization of two lectins from white-spotted charr (salvelinus leucomaenis) eggs

Hiroaki Tateno, Tomohisa Ogawa, Koji Muramoto, Hisao Kamiya, Mineo Saneyoshi

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

L-Rhamnose-binding lectins were isolated from white-spotted charr (Salvelinus leucomaenis) eggs to understand the distribution and molecular evolution of the lectins in Salmonidae. Only two L-rhamnose-binding lectins, named WCL1 and WCL3, were isolated from white-spotted charr eggs, though three lectins, named STL1, STL2, and STL3, had been obtained from steelhead trout (Oncorhynchus mykiss) eggs. The cDNAs of WCL1 and WCL3 included 1,245 and 838 bp nucleotides with open reading frames of 933 and 651 nucleotides, respectively, and encoded for the complete amino acid sequences of mature proteins consisted of 288 (WCL1) and 195 (WCL3) residues, and signal sequences of 23 and 22 residues, respectively. WCLs were composed of three (for WCL1) or two (for WCL3) tandemly repeated homologous domains, which consisted of about 95 amino acid residues, and showed 91 and 93% sequence identities to STL1 and STL3, respectively. The mRNAs of WCL1 and WCL3 were detected exclusively in liver and ovary, respectively, however, neither a protein nor mRNA corresponding to STL2 could be identified in white-spotted charr. The phylogenetic tree of the 16 sequences encoding carbohydrate recognition domains of 7 lectins from 4 species shows 5 functional clusters and their evolutional process. These results indicate that multiple L-rhamnose-binding isolectins have diverged by gene duplication and exon shuffling to play various biological roles in each species.

Original languageEnglish
Pages (from-to)1356-1365
Number of pages10
JournalBioscience, Biotechnology and Biochemistry
Volume66
Issue number6
DOIs
Publication statusPublished - 2002 Jan 1

Keywords

  • Fish egg
  • Molecular evolution
  • Rhamnose-binding lectin
  • Salmonidae
  • White-spotted charr

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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