Distribution and immunochemical specificities of fimbriae of Porphyromonas gingivalis and related bacterial species

Tomohiko Ogawa, Tetsu Mukai, Kenji Yasuda, Hidetoshi Shimauchi, Yoshihisa Toda, Shigeyuki Hamada

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Rabbit polyclonal antibody (Poly‐1) and mouse monoclonal antibodies (mAbs) TO‐11, TO‐14 and TO‐M1 specific for Porphyromonas gingivalis 381 fimbriae were prepared. Poly‐1 and the 3 mAbs were screened for their reactivity with whole cells of oral and nonoral black‐pigmented bacterial species by enzyme‐linked immunosorbent assay (ELISA) and the binding experiment using [125I] Poly‐1 and [125I] mAbs. ELISA revealed that Poly‐1 definitely reacted with whole cells of all the 11 strains of P. gingivalis tested. However, 8 of 11 P. gingivalis strains reacted with mAbs TO‐11, TO‐14 and TO‐M1. These results were confirmed by the specific binding of radiolabelled Poly‐1 and mAb TO‐11 to the 8 strains. The Mr of the fimbrial summit protein (fimbrilin) isolated and purified from P. gingivalis strains 381, BH18/10, HW24D‐1, 6/26 and OMZ 314 was 41 kDa by sodium dodecylsulfate‐polyacrylamide gel electrophoresis. It was found by immunoblotting that mAbs TO‐11 and TO‐14/TO‐M1 recognized different epitopes of fimbrial protein from P. gingivalis strains. visualized similar serotype‐specific antibody bindings to the fimbriae. These results indicate that 11 strains of P. gingivalis could be divided into at least 2 separate groups based on the immunochemical specificities of the fimbriae.

Original languageEnglish
Pages (from-to)332-340
Number of pages9
JournalOral Microbiology and Immunology
Volume6
Issue number6
DOIs
Publication statusPublished - 1991 Dec
Externally publishedYes

Keywords

  • fimbriae
  • immunochemistry
  • periodontal desease
  • porphyromonas gingivalis

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Dentistry(all)
  • Microbiology (medical)

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