Distinctive effects of arachidonic acid and docosahexaenoic acid on neural stem/progenitor cells

Nobuyuki Sakayori, Motoko Maekawa, Keiko Numayama-Tsuruta, Takashi Katura, Takahiro Moriya, Noriko Osumi

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)

Abstract

Arachidonic acid (ARA) and docosahexaenoic acid (DHA), which are the dominant polyunsaturated fatty acids in the brain, have crucial roles in brain development and function. Recent studies have shown that ARA and DHA promote postnatal neurogenesis. However, the direct effects of ARA on neural stem/progenitor cells (NSPCs) and the effects of ARA and DHA on NSPCs at the neurogenic and subsequent gliogenic stages are still unknown. Here, we analyzed the effects of ARA and DHA on neurogenesis, specifically maintenance and differentiation, using neurosphere assays. We confirmed that primary neurospheres are neurogenic NSPCs and that tertiary neurospheres are gliogenic NSPCs. Regarding the effects of ARA and DHA on neurogenic NSPCs, ARA and DHA increased the number of neurospheres, whereas neither ARA nor DHA had a detectable effect on NSPCs in the differentiation condition. In gliogenic NSPCs, DHA increased the number of neurospheres, whereas ARA had no such effect. In contrast, ARA increased the number of astrocytes, whereas DHA increased the number of neurons in the differentiation condition. These results suggest that ARA promotes the maintenance of neurogenic NSPCs and might induce the glial differentiation of gliogenic NSPCs and that DHA promotes the maintenance of both neurogenic and gliogenic NSPCs and might lead to the neuronal differentiation of gliogenic NSPCs.

Original languageEnglish
Pages (from-to)778-790
Number of pages13
JournalGenes to Cells
Volume16
Issue number7
DOIs
Publication statusPublished - 2011 Jul

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Fingerprint

Dive into the research topics of 'Distinctive effects of arachidonic acid and docosahexaenoic acid on neural stem/progenitor cells'. Together they form a unique fingerprint.

Cite this