Distinct regional distribution in the brain of messenger RNAs for the two isoforms of synaphin associated with the docking/fusion complex

T. Ishizuka, H. Saisu, S. Odani, T. Kumanishi, T. Abe

    Research output: Contribution to journalArticlepeer-review

    26 Citations (Scopus)

    Abstract

    Synaphin is a 19,000 mol. wt cytosolic protein we first found to co- purify with the docking/fusion complex crucial to neurotransmitter release from presynaptic terminals. Two isoforms of synaphin (synaphins I and 2)(also called complexins II and I, respectively) exist in the rat brain. On density gradient centrifugation of a Triton X-100 extract of brain membranes, synaphin was found to be associated with the 7S complex that contains synaptotagmin, syntaxin, synaptosomal-associated protein of 25,000 mol. wt and vesicle-associated membrane protein. A smaller complex devoid of synaphins was also identified by immunoprecipitation with a monoclonal antibody against synaptosomal-associated protein of 25,000 mol. wt. Messenger RNAs for synaphins 1 and 2 were expressed predominantly in the brain. In situ hybridization using probes specific to synaphins 1 and 2 indicated that the distribution of their mRNAs was significantly different in brain regions such as olfactory bulb, hippocampus, cerebral cortex, piriform cortex; cerebellum, thalamus and facial nuclei. These results show synaphin as a component of the 7S complex and suggest different physiological implications for the two isoforms.

    Original languageEnglish
    Pages (from-to)295-306
    Number of pages12
    JournalNeuroscience
    Volume88
    Issue number1
    DOIs
    Publication statusPublished - 1999 Jan

    Keywords

    • Docking/fusion complex
    • In situ hybridization
    • Neurotransmitter release
    • SNAREs
    • Synaphinl/complexin

    ASJC Scopus subject areas

    • Neuroscience(all)

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