Distinct regional distribution in the brain of messenger RNAs for the two isoforms of synaphin associated with the docking/fusion complex

T. Ishizuka, H. Saisu, S. Odani, T. Kumanishi, T. Abe

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Synaphin is a 19,000 mol. wt cytosolic protein we first found to co- purify with the docking/fusion complex crucial to neurotransmitter release from presynaptic terminals. Two isoforms of synaphin (synaphins I and 2)(also called complexins II and I, respectively) exist in the rat brain. On density gradient centrifugation of a Triton X-100 extract of brain membranes, synaphin was found to be associated with the 7S complex that contains synaptotagmin, syntaxin, synaptosomal-associated protein of 25,000 mol. wt and vesicle-associated membrane protein. A smaller complex devoid of synaphins was also identified by immunoprecipitation with a monoclonal antibody against synaptosomal-associated protein of 25,000 mol. wt. Messenger RNAs for synaphins 1 and 2 were expressed predominantly in the brain. In situ hybridization using probes specific to synaphins 1 and 2 indicated that the distribution of their mRNAs was significantly different in brain regions such as olfactory bulb, hippocampus, cerebral cortex, piriform cortex; cerebellum, thalamus and facial nuclei. These results show synaphin as a component of the 7S complex and suggest different physiological implications for the two isoforms.

Original languageEnglish
Pages (from-to)295-306
Number of pages12
JournalNeuroscience
Volume88
Issue number1
DOIs
Publication statusPublished - 1999 Jan
Externally publishedYes

Keywords

  • Docking/fusion complex
  • In situ hybridization
  • Neurotransmitter release
  • SNAREs
  • Synaphinl/complexin

ASJC Scopus subject areas

  • Neuroscience(all)

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