Distinct fucosylation of M cells and epithelial cells by Fut1 and Fut2, respectively, in response to intestinal environmental stress

Kazutaka Terahara, Tomonori Nochi, Masato Yoshida, Yuko Takahashi, Yoshiyuki Goto, Hirotsugu Hatai, Shiho Kurokawa, Myoung Ho Jang, Mi Na Kweon, Steven E. Domino, Takachika Hiroi, Yoshikazu Yuki, Yasuko Tsunetsugu-Yokota, Kazuo Kobayashi, Hiroshi Kiyono

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

The intestinal epithelium contains columnar epithelial cells (ECs) and M cells, and fucosylation of the apical surface of ECs and M cells is involved in distinguishing the two populations and in their response to commensal flora and environmental stress. Here, we show that fucosylated ECs (F-ECs) were induced in the mouse small intestine by the pro-inflammatory agents dextran sodium sulfate and indomethacin, in addition to an enteropathogen derived cholera toxin. Although F-ECs showed specificity for the M cell-markers, lectin Ulex europaeus agglutinin-1 and our monoclonal antibody NKM 16-2-4, these cells also retained EC-phenotypes including an affinity for the EC-marker lectin wheat germ agglutinin. Interestingly, fucosylation of Peyer's patch M cells and F-ECs was distinctly regulated by α(1,2)fucosyltransferase Fut1 and Fut2, respectively. These results indicate that Fut2-mediated F-ECs share M cell-related fucosylated molecules but maintain distinctive EC characteristics, Fut1 is, therefore, a reliable marker for M cells.

Original languageEnglish
Pages (from-to)822-828
Number of pages7
JournalBiochemical and biophysical research communications
Volume404
Issue number3
DOIs
Publication statusPublished - 2011 Jan 21
Externally publishedYes

Keywords

  • Epithelial cell
  • Fucosyltransferase
  • Intestine
  • M cell
  • Peyer's patch

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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